Issue 33, 2011
From the journal:

Chemical Communications

Targeted pH-dependent fluorescent activity-based cathepsin probes†‡

Sascha Hoogendoorn,a   Kim L. Habets,b   Solène Passemard,a   Johan Kuiper,b   Gijsbert A. van der Marel,a   Bogdan I. Floreaa  and  Herman S. Overkleeft*a  

* Corresponding authors

a Leiden Institute of Chemistry, Leiden University, 2300 RA Leiden, The Netherlands
E-mail: h.s.overkleeft@chem.leidenuniv.nl
Tel: +31(0)715274342

b Dept of Biopharmaceutics LACDR, Leiden University, 2300 RA Leiden, The Netherlands

Abstract

Bifunctional, pH-activatable BODIPY dyes were developed and incorporated in mannose cluster-containing activity-based probes for cysteine proteases. Mannose receptor-dependent uptake of the probes in dendritic cells, followed by trafficking to acidic cellular compartments resulted in fluorescence as seen by live-cell imaging, and subsequent cathepsin inhibition.

Graphical abstract: Targeted pH-dependent fluorescent activity-based cathepsin probes

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C1CC12947C
Article type
Communication
Submitted
19 мај 2011
Accepted
29 јун. 2011
First published
18 јул. 2011

Chem. Commun., 2011,47, 9363-9365

Permissions

Request permissions

Targeted pH-dependent fluorescent activity-based cathepsin probes

S. Hoogendoorn, K. L. Habets, S. Passemard, J. Kuiper, G. A. van der Marel, B. I. Florea and H. S. Overkleeft, Chem. Commun., 2011, 47, 9363 DOI: 10.1039/C1CC12947C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements