Selectivity and efficiency in the ligation of the pyrene:abasic base pair by T4 and PBCV-1 DNA ligases†
Hansol
Park 
a
and
Julianne M.
Gibbs
*a
a
and
Julianne M.
Gibbs
*a
Abstract
Remarkable selectivity was observed in the ligation of 5′-phosphate 1-pyrene nucleotide terminated strands across from an abasic lesion in a DNA-templated ligation reaction by two different ligases suggesting that pyrene-terminated strands could be used in abasic site detection. Increasing ATP concentration was critical to enhancing the selectivity for this base pair with T4 DNA ligase.
- This article is part of the themed collection: 2022 Pioneering Investigators
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