Jimei Zhou, Minxian Jia, Menghui Song, Zhiliang Huang, Alexander Steiner, Qidong An, Jianwei Ma, Zhiyin Guo, Qianqian Zhang, Huaming Sun, Craig Robertson, John Bacsa, Jianliang Xiao and Chaoqun Li

Angew Chem Int Ed, 2022, 61
DOI: 10.1002/anie.202205983

Electrochemical Benzylic C─H Sulfation beyond the O‐Sulfonation Limitation

Wei Sheng, Huanhuan Peng, Ben Gao, Chunlan Song and Jiakun Li

Angew Chem Int Ed, 2025, 64
DOI: 10.1002/anie.202507048

State-of-the-Art Biocatalysis

Joshua B. Pyser, Suman Chakrabarty, Evan O. Romero and Alison R. H. Narayan

ACS Cent. Sci., 2021, 7, 1105
DOI: 10.1021/acscentsci.1c00273

A Predictably Selective Palladium-Catalyzed Aliphatic C–H Oxygenation

Dmitry P. Lubov, Mikhail V. Shashkov, Andrey A. Nefedov and Konstantin P. Bryliakov

Org. Lett., 2023, 25, 1359
DOI: 10.1021/acs.orglett.2c04371

Ferric ion concentration-controlled aerobic photo-oxidation of benzylic C–H bond with high selectivity and conversion

Jiefan Gu, Yuting Wan, Hongfei Ma, Hongjun Zhu, Hongzhong Bu, Ying'ao Zhou, Weijian Zhang, Zheng-Guang Wu and Yufeng Li

Tetrahedron, 2021, 93, 132298
DOI: 10.1016/j.tet.2021.132298

Discovery, Characterization and Synthetic Application of a Promiscuous Nonheme Iron Biocatalyst with Dual Hydroxylase/Desaturase Activity

Hao Xu, Jidong Zhao and Hans Renata

Angew Chem Int Ed, 2024, 63
DOI: 10.1002/anie.202409143

Ru‐Catalyzed C−H Hydroxylation of Tyrosine‐Containing Di‐ and Tripeptides toward the Assembly of L‐DOPA Derivatives

Paula Andrade‐Sampedro, Jon M. Matxain and Arkaitz Correa

Adv Synth Catal, 2022, 364, 2072
DOI: 10.1002/adsc.202200234

The Transformative Power of Biocatalysis in Convergent Synthesis

Lara E. Zetzsche, Suman Chakrabarty and Alison R. H. Narayan

J. Am. Chem. Soc., 2022, 144, 5214
DOI: 10.1021/jacs.2c00224

Direct regio- and stereoselective mono- and polyoxyfunctionalization of estrone derivatives at C(sp3)-H bonds

Roman V. Ottenbacher, Denis G. Samsonenko, Andrey A. Nefedov and Konstantin P. Bryliakov

Journal of Catalysis, 2022, 415, 12
DOI: 10.1016/j.jcat.2022.09.020

Robust Light Driven Enzymatic Oxyfunctionalization via Immobilization of Unspecific Peroxygenase

Piera De Santis, Deborah Wegstein, Bastien O. Burek, Jacqueline Patzsch, Miguel Alcalde, Wolfgang Kroutil, Jonathan Z. Bloh and Selin Kara

ChemSusChem, 2023, 16
DOI: 10.1002/cssc.202300613

Regioselective Hydroxylation of Stilbenes by White‐Rot Fungal P450s Enables Preparative‐Scale Synthesis of Stilbenoids

Nico D. Fessner, Hansjörg Weber and Anton Glieder

Eur J Org Chem, 2022, 2022
DOI: 10.1002/ejoc.202101436

Novel Arylindigoids by Late‐Stage Derivatization of Biocatalytically Synthesized Dibromoindigo

Christian Schnepel, Veronica I. Dodero and Norbert Sewald

Chemistry A European J, 2021, 27, 5404
DOI: 10.1002/chem.202005191

Rhodium(III)‐Catalyzed C‐H/O2 Dual Activation and Macrocyclization: Synthesis and Evaluation of Pyrido[2,1‐a]isoindole Grafted Macrocyclic Inhibitors for Influenza H1N1

Bichao Song, Xueying Guo, Li Yang, Haiyue Yu, Xinlei Zong, Xiujuan Liu, Hao Wang, Zhongliang Xu, Zhenyang Lin and Weibo Yang

Angewandte Chemie, 2023, 135
DOI: 10.1002/ange.202218886

Dimer Stabilization by SpyTag/SpyCatcher Coupling of the Reductase Domains of a Chimeric P450 BM3 Monooxygenase from Bacillus spp. Improves its Stability, Activity, and Purification

Arabella Essert and Kathrin Castiglione

ChemBioChem, 2024, 25
DOI: 10.1002/cbic.202300650

Pd(II)-Catalyzed Hydroxyl-acyloxylation of gem-Dimethyl Groups via Dual C(sp3)–H Activation

Xiaorui Song, Zhenzhen Dong, Miaomiao Tian, Yan Qiao and Bingxian Liu

Org. Lett., 2026, 28, 3143
DOI: 10.1021/acs.orglett.6c00045

An Artificial In Vitro Metabolism to Angiopterlactone B Inspired by Traditional Retrosynthesis**

Alexander F. Kiefer, Yu‐Chang Liu, Rebecca Gummerer, Christina Jäger and Jan Deska

Angew Chem Int Ed, 2023, 62
DOI: 10.1002/anie.202301178

An Asymmetric Hydrogen Atom Transferase with an Abiological Thiophenol Cofactor

Hui Cao, Kailin Zhang, Vladimir Gorbachev, Tobias Vornholt, Kun Yu, Dongping Chen and Thomas R. Ward

J. Am. Chem. Soc., 2025, 147, 41600
DOI: 10.1021/jacs.5c12516

An Optimized System for the Study of Rieske Oxygenase‐catalyzed Hydroxylation Reactions In vitro

Michael Ernst Runda, Bastian Kremser, Fatma Feyza Özgen and Sandy Schmidt

ChemCatChem, 2023, 15
DOI: 10.1002/cctc.202300371

Engineering Unspecific Peroxygenases for Enantioselective α‐Hydroxylation of β‐Ketoesters

Zhen‐Yu Wang, Xin‐Yuan Ma, Ying Wu, Yang Liu, Guang‐Xin Lin, Xiao‐Qi Liu, Chun Zhang, Peng Chen, Yongxiang Zheng and Zhi‐Jun Jia

Angewandte Chemie, 2025, 137
DOI: 10.1002/ange.202509359

Molecular Basis of Regioselective Bridgehead C(sp3)–H Bond Hydroxylation by P450 Peroxygenase in Bicyclomycin Biosynthesis

Jun-Bin He, Lian Wu, Wenli Yuan, Hai-Xue Pan, Binju Wang and Gong-Li Tang

ACS Catal., 2026, 16, 5616
DOI: 10.1021/acscatal.5c08281

Tuning Lewis acid sites in TS-1 zeolites for hydroxylation of anisole with hydrogen peroxide

Pengyu Zhao, Zhiguang Li, Yue Zhang, Debao Cui, Qingchun Guo, Zhipeng Dong, Guodong Qi, Jun Xu and Feng Deng

Microporous and Mesoporous Materials, 2022, 335, 111840
DOI: 10.1016/j.micromeso.2022.111840

Accelerated enzyme evolution for targeted C–H oxidation in cyclic amines

Nat. Synth, 2022, 1, 921
DOI: 10.1038/s44160-022-00170-w

Engineered and artificial metalloenzymes for selective C–H functionalization

Xinkun Ren and Rudi Fasan

Current Opinion in Green and Sustainable Chemistry, 2021, 31, 100494
DOI: 10.1016/j.cogsc.2021.100494

tert‐Butyl as a Functional Group: Non‐Directed Catalytic Hydroxylation of Sterically Congested Primary C−H Bonds

Siu‐Chung Chan, Andrea Palone, Massimo Bietti and Miquel Costas

Angewandte Chemie, 2024, 136
DOI: 10.1002/ange.202402858

Molecular and Computational Bases for Spirofuranone Formation in Setosusin Biosynthesis

Xingxing Wei, Taro Matsuyama, Hajime Sato, Dexiu Yan, Pui Man Chan, Kazunori Miyamoto, Masanobu Uchiyama and Yudai Matsuda

J. Am. Chem. Soc., 2021, 143, 17708
DOI: 10.1021/jacs.1c08336

Characterization of an Extradiol Dioxygenase Using Integrating Cavity Spectrophotometry

Thomas Benning, Hannah R. Zwiefelhofer, Cadence F. Cordova, Grace C. Rooney and Tyler J. Doyon

ChemBioChem, 2025, 26
DOI: 10.1002/cbic.202500563

Enantioselective High‐Throughput Assay Showcased for the Identification of (R)‐ as well as (S)‐Selective Unspecific Peroxygenases for C−H Oxidation

Alexander Swoboda, Lukas Johannes Pfeifenberger, Zerina Duhović, Moritz Bürgler, Isabel Oroz‐Guinea, Klara Bangert, Florian Weißensteiner, Lena Parigger, Katharina Ebner, Anton Glieder and Wolfgang Kroutil

Angew Chem Int Ed, 2023, 62
DOI: 10.1002/anie.202312721

Unspecific peroxygenases and P450-derived peroxygenases as green biocatalysts driven by H2O2 for sustainable applications: A review

Jikai Gao, Wenjing Wang, Ziyang Yin, Jiaying Ai, Fuping Lu, Hui-Min Qin and Shuhong Mao

International Journal of Biological Macromolecules, 2025, 322, 146548
DOI: 10.1016/j.ijbiomac.2025.146548

Catalytic Asymmetric Oxidative Coupling between C(sp3)–H Bonds and Carboxylic Acids

Xian-Ming Liu, Fu Li, Tongkun Wang, Ling Dai, Yin Yang, Neng-Quan Jiang, Li-Yuan Xue, Jing-Yuan Liu, Xiao-Song Xue, Li-Jun Xiao and Qi-Lin Zhou

J. Am. Chem. Soc., 2025, 147, 627
DOI: 10.1021/jacs.4c12544

Regiodivergent and Enantioselective Hydroxylation of C−H bonds by Synergistic Use of Protein Engineering and Exogenous Dual‐Functional Small Molecules

Jie Chen, Sheng Dong, Wenhan Fang, Yiping Jiang, Zhifeng Chen, Xiangquan Qin, Cong Wang, Haifeng Zhou, Longyi Jin, Yingang Feng, Binju Wang and Zhiqi Cong

Angew Chem Int Ed, 2023, 62
DOI: 10.1002/anie.202215088

Photobiocatalytic Oxyfunctionalization with High Reaction Rate using a Baeyer–Villiger Monooxygenase from Burkholderia xenovorans in Metabolically Engineered Cyanobacteria

Elif Erdem, Lenny Malihan-Yap, Leen Assil-Companioni, Hanna Grimm, Giovanni Davide Barone, Carole Serveau-Avesque, Agnes Amouric, Katia Duquesne, Véronique de Berardinis, Yagut Allahverdiyeva, Véronique Alphand and Robert Kourist

ACS Catal., 2022, 12, 66
DOI: 10.1021/acscatal.1c04555

Electrostatic interaction and regioselectivity enhancement in proline cis-4-hydroxylase for L-pipecolic acid hydroxylation

Fujun Huang, Junyu Wang, Lufei Zhang, Qimo Yin, Yunlin Shi, Hai Ye, Bin Wu, Hongli Wu, Fei Cao and Ping Wei

Molecular Catalysis, 2024, 553, 113762
DOI: 10.1016/j.mcat.2023.113762

High-Throughput 19F NMR Chiral Analysis for Screening and Directed Evolution of Imine Reductases

Shucheng Song, Chenyang Wang, Wenjing Bao, Zhenchuang Xu, Jian Wu, Liang Lin and Yanchuan Zhao

ACS Cent. Sci., 2025, 11, 1094
DOI: 10.1021/acscentsci.5c00498

Relay Oxidation with Engineered P450 BM3 and Aldo-Keto Reductase: Chemoenzymatic Synthesis of Erythrina Alkaloids

Longxing Wang, Lili Zhu, Lingyong Wang, Min Shi, Huilei Yu and Ran Hong

ACS Catal., 2026, 16, 7025
DOI: 10.1021/acscatal.6c00910

Catalytic Enantioselective Hydroxylation of Tertiary Propargylic C(sp3)–H Bonds in Acyclic Systems: a Kinetic Resolution Study

Min Cao, Hongliang Wang, Fangao Hou, Yuhang Zhu, Qianqian Liu, Chen-Ho Tung and Lei Liu

J. Am. Chem. Soc., 2024, 146, 18396
DOI: 10.1021/jacs.4c03610

Computational Redesign and Mechanistic Insights into P450BM3 Enable Regioselective C–H Hydroxylation of Structurally Diverse Steroids

Qi Ye, Zhihao Li, Ying Cui, Hongjia Wei, Youqiao Zhang, Fan Zhao, Fuju Wang, Yongbo Song, Weizhuo Xu and Jinghai Zhang

ACS Catal., 2026, 16, 2745
DOI: 10.1021/acscatal.5c08382

Palladium catalyzed selective benzylic C‒H alkoxylation

Dmitry P. Lubov, Konstantin S. Ivanov, Andrey A. Nefedov and Konstantin P. Bryliakov

Journal of Catalysis, 2026, 459, 116895
DOI: 10.1016/j.jcat.2026.116895

A tautomeric ligand enables directed C‒H hydroxylation with molecular oxygen

Zhen Li, Zhen Wang, Nikita Chekshin, Shaoqun Qian, Jennifer X. Qiao, Peter T. Cheng, Kap-Sun Yeung, William R. Ewing and Jin-Quan Yu

Science, 2021, 372, 1452
DOI: 10.1126/science.abg2362

C–H functionalization reactions catalyzed by artificial metalloenzymes

Kun Yu and Thomas R. Ward

Journal of Inorganic Biochemistry, 2024, 258, 112621
DOI: 10.1016/j.jinorgbio.2024.112621

Computationally Designed Peroxygenases That Exhibit Diverse and Selective Terpene Oxyfunctionalization

Judith Münch, Jordi Soler, Ofir Gildor-Cristal, Sarel J. Fleishman, Marc Garcia-Borràs and Martin J. Weissenborn

ACS Catal., 2025, 15, 12741
DOI: 10.1021/acscatal.5c02412

Recent Advances in Biocatalysis for Drug Synthesis

Alina Kinner, Philipp Nerke, Regine Siedentop, Till Steinmetz, Thomas Classen, Katrin Rosenthal, Markus Nett, Jörg Pietruszka and Stephan Lütz

Biomedicines, 2022, 10, 964
DOI: 10.3390/biomedicines10050964

The Role of the Unusual 2-Tyr-1-carboxylate Nonheme Iron Motif in the Mechanism of N,N-Dimethylformamidase

David W. Kastner, Clorice R. Reinhardt, Husain Adamji, Terence S. Oscar-Okpala, Ilia Kevlishvili, Yuriy Román-Leshkov and Heather J. Kulik

ACS Catal., 2025, 15, 12822
DOI: 10.1021/acscatal.5c03335

Recent Progress in Electrochemical Late-Stage C(sp2)–H Functionalization

Yanjun Li, Jinbin Zhu, Jiandong Liu and Lutz Ackermann

Synthesis, 2026, 58, 251
DOI: 10.1055/a-2735-9063

A H2O2 generation-detection-regulation integrated platform for boosting the efficiency of peroxygenase-catalysed C–H oxidative hydroxylation

Xiaowang Zhang, Zhuotao Tan, Mengjiao Xu, Wei Zhuang, Hanjie Ying, Zhenyu Chu and Chenjie Zhu

Green Synthesis and Catalysis, 2024, 5, 153
DOI: 10.1016/j.gresc.2023.11.006

Oxidation of alcohols to aldehydes with bromoisobutyrate and dimethyl sulfoxide

Fei-Yue Wu, Xiao-Hui Chen, Hai-Mei Zhou, Jia-Qin Li and Hai-Lei Cui

Tetrahedron Letters, 2021, 83, 153428
DOI: 10.1016/j.tetlet.2021.153428

Late-stage C–H functionalization offers new opportunities in drug discovery

Lucas Guillemard, Nikolaos Kaplaneris, Lutz Ackermann and Magnus J. Johansson

Nat Rev Chem, 2021, 5, 522
DOI: 10.1038/s41570-021-00300-6

C–H bond functionalization reactions enabled by photobiocatalytic cascades

Yuanying Li, Bo Yuan, Zhoutong Sun and Wuyuan Zhang

Green Synthesis and Catalysis, 2021, 2, 267
DOI: 10.1016/j.gresc.2021.06.001

Mechanisms of C(sp3)–H and C=C selective oxidative heterofunctionalizations by non-heme Fe and Mn mimics of oxygenase enzymes

Konstantin P. Bryliakov

Coordination Chemistry Reviews, 2024, 508, 215793
DOI: 10.1016/j.ccr.2024.215793

Bioinspired Skeletal Reorganization Approach for the Synthesis of Steroid Natural Products

Yu Wang and Jinghan Gui

Acc. Chem. Res., 2024, 57, 568
DOI: 10.1021/acs.accounts.3c00716

Versatile Biocatalytic C(sp3)−H Oxyfunctionalization for the Site‐ Selective and Stereodivergent Synthesis of α‐ and β‐Hydroxy Acids

Yingle Mao, Weijie Zhang, Zunyun Fu, Yanqiong Liu, Lin Chen, Xin Lian, Dan Zhuo, Jiewei Wu, Mingyue Zheng and Cangsong Liao

Angew Chem Int Ed, 2023, 62
DOI: 10.1002/anie.202305250

Mn-Catalyzed Regio- and Stereoselective C(sp3)–H Lactonization of Carboxylic Acids with H2O2

Vladimir I. Kurganskiy, Anna A. Bryliakova, Alexander G. Medvedev, Mikhail V. Shashkov and Konstantin P. Bryliakov

ACS Catal., 2025, 15, 14938
DOI: 10.1021/acscatal.5c03762

Biocatalytic approach for the synthesis of chiral alcohols for the development of pharmaceutical intermediates and other industrial applications: A review

Mohd Naim, Mohd Fazli Mohammat, Putri Nur Arina Mohd Ariff and Mohamad Hekarl Uzir

Enzyme and Microbial Technology, 2024, 180, 110483
DOI: 10.1016/j.enzmictec.2024.110483

Direct C–H Hydroxylation of N-Heteroarenes and Benzenes via Base-Catalyzed Halogen Transfer

Kendelyn I. Bone, Thomas R. Puleo and Jeffrey S. Bandar

J. Am. Chem. Soc., 2024, 146, 9755
DOI: 10.1021/jacs.3c14058

Highly Stereoselective Biocatalytic One-Pot Synthesis of Chiral Saturated Oxygen Heterocycles by Integration of a Biosynthetic Heterocyclase into Multiple-Enzyme Cascades

Theresa Roß-Taschner, Sebastian Derra, Jörg Stang, Luca Schlotte, Anthony Putratama and Frank Hahn

ACS Catal., 2024, 14, 13420
DOI: 10.1021/acscatal.4c03692

Selective C(sp3)–H Oxyfunctionalizations in the Presence of Ru, Rh, and Pd Based Catalysts

Dmitry P. Lubov and Konstantin P. Bryliakov

Coordination Chemistry Reviews, 2026, 562, 217891
DOI: 10.1016/j.ccr.2026.217891

Lossen Rearrangement vs C–N Reductive Elimination Enabled by Rh(III)-Catalyzed C–H Activation/Selective Lactone Ring-Opening: Chemodivergent Synthesis of Quinolinones and Dihydroisoquinolinones

Mengyao Bian, Hamdulla Mawjuda, Hui Gao, Huiying Xu, Zhi Zhou and Wei Yi

Org. Lett., 2020, 22, 9677
DOI: 10.1021/acs.orglett.0c03734

Engineering Unspecific Peroxygenases for Enantioselective α‐Hydroxylation of β‐Ketoesters

Zhen‐Yu Wang, Xin‐Yuan Ma, Ying Wu, Yang Liu, Guang‐Xin Lin, Xiao‐Qi Liu, Chun Zhang, Peng Chen, Yongxiang Zheng and Zhi‐Jun Jia

Angew Chem Int Ed, 2025, 64
DOI: 10.1002/anie.202509359

Oxygenating Biocatalysts for Hydroxyl Functionalisation in Drug Discovery and Development

Sacha N. Charlton and Martin A. Hayes

ChemMedChem, 2022, 17
DOI: 10.1002/cmdc.202200115

Development of Scalable Processes with Underutilized Biocatalyst Classes

Ryan M. Phelan, Michael J. Abrahamson, Jesse T. C. Brown, Ruijie K. Zhang and Christian R. Zwick

Org. Process Res. Dev., 2022, 26, 1944
DOI: 10.1021/acs.oprd.1c00467

Regioselective and enantioselective propargylic hydroxylations catalyzed by P450tol monooxygenases

Xu Deng, Cheng-Cheng Song, Wen-Jing Gu, Yu-Jie Wang, Lu Feng, Xiao-Jian Zhou, Ming-Qiang Zhou, Wei-Cheng Yuan and Yong-Zheng Chen

Bioresour. Bioprocess., 2024, 11
DOI: 10.1186/s40643-024-00771-7

Transition Metal-Catalyzed Direct Stereoselective Oxygenations of C(sp3)–H Groups

Konstantin P. Bryliakov

ACS Catal., 2023, 13, 10770
DOI: 10.1021/acscatal.3c02282

Chemoenzymatic Total Synthesis of Natural Products

Suman Chakrabarty, Evan O. Romero, Joshua B. Pyser, Jessica A. Yazarians and Alison R. H. Narayan

Acc. Chem. Res., 2021, 54, 1374
DOI: 10.1021/acs.accounts.0c00810

New Trends and Future Opportunities in the Enzymatic Formation of C−C, C−N, and C−O bonds

Jack J. Sangster, James R. Marshall, Nicholas J. Turner and Juan Mangas‐Sanchez

ChemBioChem, 2022, 23
DOI: 10.1002/cbic.202100464

Enzymatic Regio- and Enantioselective C–H Oxyfunctionalization of Fatty Acids

Hao Chen, Mengfei Huang, Wenliang Yan, Wen-Ju Bai and Xiqing Wang

ACS Catal., 2021, 11, 10625
DOI: 10.1021/acscatal.1c03292

Electrochemical Benzylic C─H Sulfation beyond the O‐Sulfonation Limitation

Wei Sheng, Huanhuan Peng, Ben Gao, Chunlan Song and Jiakun Li

Angewandte Chemie, 2025, 137
DOI: 10.1002/ange.202507048

Bromoacetic Acid-Promoted Nonheme Manganese-Catalyzed Alkane Hydroxylation Inspired by α-Ketoglutarate-Dependent Oxygenases

Jie Chen, Jinping Yao, Xiao-Xi Li, Yan Wang, Wenxun Song, Kyung-Bin Cho, Yong-Min Lee, Wonwoo Nam and Bin Wang

ACS Catal., 2022, 12, 6756
DOI: 10.1021/acscatal.2c01096

Advances in enzymatic oxyfunctionalization of aliphatic compounds

Carmen Aranda, Juan Carro, Alejandro González-Benjumea, Esteban D. Babot, Andrés Olmedo, Dolores Linde, Angel T. Martínez and Ana Gutiérrez

Biotechnology Advances, 2021, 51, 107703
DOI: 10.1016/j.biotechadv.2021.107703

YfeX – A New Platform for Carbene Transferase Development with High Intrinsic Reactivity

Victor Sosa Alfaro, Sodiq O. Waheed, Hannah Palomino, Anja Knorrscheidt, Martin Weissenborn, Christo Z. Christov and Nicolai Lehnert

Chemistry A European J, 2022, 28
DOI: 10.1002/chem.202201474

Preparative Production of Functionalized (N- and O-Heterocyclic) Polycyclic Aromatic Hydrocarbons by Human Cytochrome P450 3A4 in a Bioreactor

Matic Srdič, Nico D. Fessner, Deniz Yildiz, Anton Glieder, Markus Spiertz and Ulrich Schwaneberg

Biomolecules, 2022, 12, 153
DOI: 10.3390/biom12020153

Regiodivergent and Enantioselective Hydroxylation of C−H bonds by Synergistic Use of Protein Engineering and Exogenous Dual‐Functional Small Molecules

Jie Chen, Sheng Dong, Wenhan Fang, Yiping Jiang, Zhifeng Chen, Xiangquan Qin, Cong Wang, Haifeng Zhou, Longyi Jin, Yingang Feng, Binju Wang and Zhiqi Cong

Angewandte Chemie, 2023, 135
DOI: 10.1002/ange.202215088

Medicinal Chemistry for Sustainable Development

Luana da S.M. Forezi, Patricia G. Ferreira, Alcione Silva de Carvalho, Fernando de C. da Silva and Vitor F. Ferreira

CTMC, 2023, 23, 957
DOI: 10.2174/1568026623666230517114621

Site and Enantioselective Aliphatic C−H Oxidation with Bioinspired Chiral Complexes

Miquel Costas

The Chemical Record, 2021, 21, 4000
DOI: 10.1002/tcr.202100227

Power of Biocatalysis for Organic Synthesis

Christoph K. Winkler, Joerg H. Schrittwieser and Wolfgang Kroutil

ACS Cent. Sci., 2021, 7, 55
DOI: 10.1021/acscentsci.0c01496

Organoplatinum Chemistry Related to Alkane Oxidation: The Effect of a Nitro Substituent in Ligands Having an Appended Phenol Group

Anwar Abo-Amer, Mohamed E. Moustafa, Paul D. Boyle and Richard J. Puddephatt

Inorganics, 2024, 12, 32
DOI: 10.3390/inorganics12010032

Synthesis of Difluorinated Dihydrobenzo[de]chromenes via Rh(III)‐Catalysed C‐H Couplings of 1‐Naphthols with Gem‐Difluoromethylene Alkynes

Liping Li, Xiuhua Zhong, Jiali Xu, Hui Gao, Zhi Zhou and Wei Yi

Adv Synth Catal, 2021, 363, 1352
DOI: 10.1002/adsc.202001475

Site- and enantioselective allylic and propargylic C–H oxidation enabled by copper-based biomimetic catalysis

Honggang Zhang, Yibo Zhou, Tilong Yang, Jingui Wu, Pinhong Chen, Zhenyang Lin and Guosheng Liu

Nat Catal, 2025, 8, 58
DOI: 10.1038/s41929-024-01276-4

Biocatalysis

Elizabeth L. Bell, William Finnigan, Scott P. France, Anthony P. Green, Martin A. Hayes, Lorna J. Hepworth, Sarah L. Lovelock, Haruka Niikura, Sílvia Osuna, Elvira Romero, Katherine S. Ryan, Nicholas J. Turner and Sabine L. Flitsch

Nat Rev Methods Primers, 2021, 1
DOI: 10.1038/s43586-021-00044-z

Practical Enzymatic Production of Carbocycles

Weijin Wang, Douglass F. Taber and Hans Renata

Chemistry A European J, 2021, 27, 11773
DOI: 10.1002/chem.202101232

Biocatalytic enantioselective γ-C–H lactonization of aliphatic carboxylic acids

Qian Ma, Wenhao Shan, Xuan Chu, Haochen Xu, Ziyi Chen, Fudong Li, Jie-Lou Liao, Chao He, Wen-Ju Bai and Xiqing Wang

Nat. Synth, 2023, 3, 123
DOI: 10.1038/s44160-023-00427-y

Photo- and Copper-Catalyzed Enantioselective Oxidation of Benzylic C(sp3)–H Bonds

Fu Li, Xian-Ming Liu, Jia-Bin Pan, Ling Dai, Yin Yang, Li-Jun Xiao, Chao Fan and Qi-Lin Zhou

J. Am. Chem. Soc., 2025, 147, 19093
DOI: 10.1021/jacs.5c04142

Rhodium(III)‐Catalyzed C‐H/O2 Dual Activation and Macrocyclization: Synthesis and Evaluation of Pyrido[2,1‐a]isoindole Grafted Macrocyclic Inhibitors for Influenza H1N1

Bichao Song, Xueying Guo, Li Yang, Haiyue Yu, Xinlei Zong, Xiujuan Liu, Hao Wang, Zhongliang Xu, Zhenyang Lin and Weibo Yang

Angew Chem Int Ed, 2023, 62
DOI: 10.1002/anie.202218886

Chemoselective Oxyfunctionalization of Functionalized Benzylic Compounds with a Manganese Catalyst

Jimei Zhou, Minxian Jia, Menghui Song, Zhiliang Huang, Alexander Steiner, Qidong An, Jianwei Ma, Zhiyin Guo, Qianqian Zhang, Huaming Sun, Craig Robertson, John Bacsa, Jianliang Xiao and Chaoqun Li

Angewandte Chemie, 2022, 134
DOI: 10.1002/ange.202205983

Catalyst- and Oxidizing Reagent-Free Electrochemical Benzylic C(sp3)–H Oxidation of Phenol Derivatives

Zhong-Wei Hou, Ming-Ming Zhang, Wen-Chao Yang and Lei Wang

J. Org. Chem., 2022, 87, 7806
DOI: 10.1021/acs.joc.2c00455

Ein künstlicher in vitro‐Stoffwechsel zu Angiopterlacton B nach dem Vorbild traditioneller Retrosynthese**

Alexander F. Kiefer, Yu‐Chang Liu, Rebecca Gummerer, Christina Jäger and Jan Deska

Angewandte Chemie, 2023, 135
DOI: 10.1002/ange.202301178

Empowering Precision C–H Functionalization: Advances in Peroxygenase Engineering

Shuaiqi Meng, Zhongyu Li, Yuncheng Du, Xiao Cheng, Binbin Nian, Siyu Fu, Xiaofei Song and Haiyang Cui

Angew Chem Int Ed, 2026
DOI: 10.1002/anie.202525086

Alcohol dehydrogenases catalyzed asymmetric reductions of “difficult-to-reduce” ketones and the involved mechanism of stereoselective recognition and regulation

Xin Su, Jie Gu, Yan Xu and Yao Nie

Critical Reviews in Biotechnology, 2026, 46, 655
DOI: 10.1080/07388551.2026.2638569

Peroxygenase-Driven Ethylbenzene Hydroxylation in a Rotating Bed Reactor

Markus Hobisch, Piera De Santis, Simona Serban, Alessandra Basso, Emil Byström and Selin Kara

Org. Process Res. Dev., 2022, 26, 2761
DOI: 10.1021/acs.oprd.2c00211

Enantiodivergent Synthesis of Halohydrins by Engineering P450DA Monooxygenases

Xiao-Jian Zhou, Mao-Yao Wang, Ling Zhao, Yu-Qi He, Zhong-Qiang Wang, Jia-Jing Li, Guo-Zhong Deng, Nan-Wei Wan and Yong-Zheng Chen

ACS Catal., 2023, 13, 15948
DOI: 10.1021/acscatal.3c04742

Discovery, Characterization and Synthetic Application of a Promiscuous Nonheme Iron Biocatalyst with Dual Hydroxylase/Desaturase Activity

Hao Xu, Jidong Zhao and Hans Renata

Angewandte Chemie, 2024, 136
DOI: 10.1002/ange.202409143

Shortening Synthetic Routes to Small Molecule Active Pharmaceutical Ingredients Employing Biocatalytic Methods

Stefan Simić, Erna Zukić, Luca Schmermund, Kurt Faber, Christoph K. Winkler and Wolfgang Kroutil

Chem. Rev., 2022, 122, 1052
DOI: 10.1021/acs.chemrev.1c00574

The Stereoselective Oxidation of para‐Substituted Benzenes by a Cytochrome P450 Biocatalyst

Rebecca R. Chao, Ian C.‐K. Lau, Tom Coleman, Luke R. Churchman, Stella A. Child, Joel H. Z. Lee, John B. Bruning, James J. De Voss and Stephen G. Bell

Chemistry A European J, 2021, 27, 14765
DOI: 10.1002/chem.202102757

Biocatalytic Enantioselective β‐Hydroxylation of Unactivated C−H Bonds in Aliphatic Carboxylic Acids

Kun Zhang, Aiqin Yu, Xuan Chu, Fudong Li, Juan Liu, Lin Liu, Wen‐Ju Bai, Chao He and Xiqing Wang

Angew Chem Int Ed, 2022, 61
DOI: 10.1002/anie.202204290

Engineered Bacterial Flavin‐Dependent Monooxygenases for the Regiospecific Hydroxylation of Polycyclic Phenols

Susann Herrmann, Martin Dippe, Pascal Pecher, Evelyn Funke, Markus Pietzsch and Ludger A. Wessjohann

ChemBioChem, 2022, 23
DOI: 10.1002/cbic.202100480

Engineering Peroxygenase Activity into Cytochrome P450 Monooxygenases through Modification of the Oxygen Binding Region

Matthew N. Podgorski, Jinia Akter, Luke R. Churchman, John B. Bruning, James J. De Voss and Stephen G. Bell

ACS Catal., 2024, 14, 7426
DOI: 10.1021/acscatal.4c01326

Engineering metalloenzymes for new-to-nature carbene and nitrene transfer biocatalysis

Wenjin Pan, Xinlong Fan, Wantong Jiang, Sirui Xin, Ningzhi Wang, Qian Wang, Keyang Yu and Xinkun Ren

Chinese Journal of Catalysis, 2025, 72, 4
DOI: 10.1016/S1872-2067(25)64659-6

Scalable and Stereoselective C–H Bond Hydroxylation of Steroids Using a Designer Heme-Thiolate Peroxygenase Biocatalyst

Oghenesivwe Osiebe, Eva F. Hayball, Nikita Yevstigneyev, Annalise M. Abbott and Stephen G. Bell

J. Org. Chem., 2026, 91, 1165
DOI: 10.1021/acs.joc.5c02818

A Miniaturized Chemical High Throughput Experimentation Plate to Enable Late-Stage Oxidation Screening and Scale-up

Javier Izquierdo-Ferrer, Mary E. Bellizzi, Noah Tu, Andrew Bogdan and Ying Wang

ACS Med. Chem. Lett., 2025, 16, 916
DOI: 10.1021/acsmedchemlett.5c00175

Enzymatic epoxidation strategies for the stereoselective synthesis of chiral epoxides

Vytautas Petkevičius, Carolin Mügge, Dirk Tischler and Rolandas Meškys

Cell Reports Physical Science, 2025, 6, 102794
DOI: 10.1016/j.xcrp.2025.102794

Three distinct strategies lead to programmable aliphatic C−H oxidation in bicyclomycin biosynthesis

Lian Wu, Jun-Bin He, Wanqing Wei, Hai-Xue Pan, Xin Wang, Sheng Yang, Yong Liang, Gong-Li Tang and Jiahai Zhou

Nat Commun, 2025, 16
DOI: 10.1038/s41467-025-58997-8

Versatile Biocatalytic C(sp3)−H Oxyfunctionalization for the Site‐ Selective and Stereodivergent Synthesis of α‐ and β‐Hydroxy Acids

Yingle Mao, Weijie Zhang, Zunyun Fu, Yanqiong Liu, Lin Chen, Xin Lian, Dan Zhuo, Jiewei Wu, Mingyue Zheng and Cangsong Liao

Angewandte Chemie, 2023, 135
DOI: 10.1002/ange.202305250

Molecular Insights into the Regioselectivity of the Fe(II)/2-Ketoglutarate-Dependent Dioxygenase-Catalyzed C–H Hydroxylation of Amino Acids

Lunjie Wu, Jianhong An, Xiaoran Jing, Chun-Chi Chen, Longhai Dai, Yan Xu, Weidong Liu, Rey-Ting Guo and Yao Nie

ACS Catal., 2022, 12, 11586
DOI: 10.1021/acscatal.2c03106

Advanced Electroanalysis for Electrosynthesis

Monica Brachi, Wassim El Housseini, Kevin Beaver, Rohit Jadhav, Ashwini Dantanarayana, Dylan G. Boucher and Shelley D. Minteer

ACS Org. Inorg. Au, 2024, 4, 141
DOI: 10.1021/acsorginorgau.3c00051

Triaryl Carbenium Ion Pair Mediated Electrocatalytic Benzylic C−H Oxygenation in Air

Zhenguo Zhang, Yongheng Lv, Liang Ji, Peng Chen, Shuyan Han, Yufei Zhu, Lanyang Li, Zhenhua Jia and Teck‐Peng Loh

Angewandte Chemie, 2024, 136
DOI: 10.1002/ange.202406588

Modulating regioselectivity of CYP107J3-catalyzed isophorone hydroxylation by disrupting the hydrophobic balance of the substrate binding pocket

Meng Dai, Xiaojuan Yu, Xiaogang Peng, Zhiyong Guo, Huili Yu and Aitao Li

Green Synthesis and Catalysis, 2026, 7, 198
DOI: 10.1016/j.gresc.2024.05.007

Indigo Formation as a Predictor of Non-Native Aromatic Hydroxylation in Cytochrome P450 BM3

Jonathan N. Besna, Douglas J. Fansher, Donya Valikhani, Olivier Rousseau, Claudèle Lemay-St-Denis, Guillaume Lamoureux and Joelle N. Pelletier

ACS Catal., 2026, 16, 404
DOI: 10.1021/acscatal.5c06523

Photoinduced Copper-Catalyzed Enantioselective Allylic C(sp3)–H Oxygenation of Acyclic Terminal Olefins Enabled by SphenBOX

Xu-Kuan Qi, Qiang Dai, Yangyi Gu, Kwan Shing Lau, Herman H. Y. Sung, Ian D. Williams, Zhenyang Lin, Chaoshen Zhang and Jianwei Sun

J. Am. Chem. Soc., 2025, 147, 20225
DOI: 10.1021/jacs.5c06136

Stereoselective construction of β-, γ- and δ-lactam rings via enzymatic C–H amidation

Satyajit Roy, David A. Vargas, Pengchen Ma, Arkajyoti Sengupta, Ledong Zhu, K. N. Houk and Rudi Fasan

Nat Catal, 2023, 7, 65
DOI: 10.1038/s41929-023-01068-2

Mechanochemical C−X/C−H Functionalization: An Alternative Strategic Access to Pharmaceuticals

Xinjie Yang, Chongyang Wu, Weike Su and Jingbo Yu

Eur J Org Chem, 2022, 2022
DOI: 10.1002/ejoc.202101440

Photobiocatalytic benzylic C–H acylation enabled by the synergy of a thiamine-dependent enzyme, an organophotocatalyst and hydrogen-atom transfer

Xichao Peng, Jianqiang Feng, Fulu Liu, Wen-Zheng Xie, Hailong Sun, Yuanyuan Xu, Yang Ming, Zhongqiu Xing, Yu Zheng, Binju Wang, Yi-Tao Long and Xiaoqiang Huang

Nat. Synth, 2025, 4, 1453
DOI: 10.1038/s44160-025-00866-9

Biocatalytic oxidative cross-coupling reactions for biaryl bond formation

Lara E. Zetzsche, Jessica A. Yazarians, Suman Chakrabarty, Meagan E. Hinze, Lauren A. M. Murray, April L. Lukowski, Leo A. Joyce and Alison R. H. Narayan

Nature, 2022, 603, 79
DOI: 10.1038/s41586-021-04365-7

Heterogeneous Photocatalysis for Late‐Stage Functionalization: A Sustainable Frontier for Complex Molecule Synthesis

Prakash Kumar Sahoo and Shoubhik Das

ChemSusChem, 2026, 19
DOI: 10.1002/cssc.202502495

Biocatalytic Enantioselective β‐Hydroxylation of Unactivated C−H Bonds in Aliphatic Carboxylic Acids

Kun Zhang, Aiqin Yu, Xuan Chu, Fudong Li, Juan Liu, Lin Liu, Wen‐Ju Bai, Chao He and Xiqing Wang

Angewandte Chemie, 2022, 134
DOI: 10.1002/ange.202204290

Scalable Electrochemical Aerobic Oxygenation of Indoles to Isatins without Electron Transfer Mediators by Merging with an Oxygen Reduction Reaction

Weihui Zhuang, Jiaqi Zhang, Cheng Ma, James S. Wright, Xiaofeng Zhang, Shao-Fei Ni and Qiufeng Huang

Org. Lett., 2022, 24, 4229
DOI: 10.1021/acs.orglett.2c01545

Biocatalytic Stereodivergent Construction of Axially Chiral Tri- and Tetrasubstituted Allenols via Desymmetric Hydroxylation

Xiao-Jian Zhou, Cheng-Cheng Song, An-Ni Wang, Mei-Xia Wang, Bei-Bei Liu, Lu Feng and Yong-Zheng Chen

J. Am. Chem. Soc., 2026, 148, 12447
DOI: 10.1021/jacs.5c08146

Emerging Chemical Diversity and Potential Applications of Enzymes in the DMSO Reductase Superfamily

Chi (Chip) Le, Minwoo Bae, Sina Kiamehr and Emily P. Balskus

Annu. Rev. Biochem., 2022, 91, 475
DOI: 10.1146/annurev-biochem-032620-110804

Electrochemical Late-Stage Functionalization

Yulei Wang, Suman Dana, Hao Long, Yang Xu, Yanjun Li, Nikolaos Kaplaneris and Lutz Ackermann

Chem. Rev., 2023, 123, 11269
DOI: 10.1021/acs.chemrev.3c00158

Discovery and Heterologous Expression of Unspecific Peroxygenases

Katharina Ebner, Lukas J. Pfeifenberger, Claudia Rinnofner, Veronika Schusterbauer, Anton Glieder and Margit Winkler

Catalysts, 2023, 13, 206
DOI: 10.3390/catal13010206

Modular chemoenzymatic synthesis of ten fusicoccane diterpenoids

Yanlong Jiang and Hans Renata

Nat. Chem., 2024, 16, 1531
DOI: 10.1038/s41557-024-01533-w

Exploring Chemical Modifications of Aromatic Amino Acid Residues in Peptides

Susanta Bhunia, Manasa Purushotham, Ganesh Karan, Bishwajit Paul and Modhu Sudan Maji

Synthesis, 2023, 55, 3701
DOI: 10.1055/a-2091-8062

Mn aminopyridine oxidase mimics: Switching between biosynthetic-like and xenobiotic regioselectivity in C H oxidation of (-)-ambroxide

Roman V. Ottenbacher, Denis G. Samsonenko, Andrey A. Nefedov, Evgenii P. Talsi and Konstantin P. Bryliakov

Journal of Catalysis, 2021, 399, 224
DOI: 10.1016/j.jcat.2021.05.014

Highly Enantioselective Catalytic Lactonization at Nonactivated Primary and Secondary γ-C–H Bonds

Arnau Call, Giorgio Capocasa, Andrea Palone, Laia Vicens, Eric Aparicio, Najoua Choukairi Afailal, Nikos Siakavaras, Maria Eugènia López Saló, Massimo Bietti and Miquel Costas

J. Am. Chem. Soc., 2023, 145, 18094
DOI: 10.1021/jacs.3c06231

Toward Sustainability: Intensification of Light‐Driven Whole Cell Biocatalysis

Lenny Malihan‐Yap, Pablo Domínguez de Maria and Robert Kourist

ChemBioChem, 2026, 27
DOI: 10.1002/cbic.70320

tert‐Butyl as a Functional Group: Non‐Directed Catalytic Hydroxylation of Sterically Congested Primary C−H Bonds

Siu‐Chung Chan, Andrea Palone, Massimo Bietti and Miquel Costas

Angew Chem Int Ed, 2024, 63
DOI: 10.1002/anie.202402858

Computational-Aided Engineering of a Selective Unspecific Peroxygenase toward Enantiodivergent β-Ionone Hydroxylation

Judith Münch, Jordi Soler, Nicole Hünecke, Dominik Homann, Marc Garcia-Borràs and Martin J. Weissenborn

ACS Catal., 2023, 13, 8963
DOI: 10.1021/acscatal.3c00702

Binding of Dual-Function Hybridized Metal–Organic Capsules to Enzymes for Cascade Catalysis

Junkai Cai, Liang Zhao, Yanan Li, Cheng He, Chong Wang and Chunying Duan

JACS Au, 2022, 2, 1736
DOI: 10.1021/jacsau.2c00322

A Porphyrin-Faced Zn8L6 Cage for Selective Oxidation of C(sp3)–H Bonds and Sulfides

Haoyu Zhai, Zhong Wei, Xu Jing and Chunying Duan

Inorg. Chem., 2024, 63, 14375
DOI: 10.1021/acs.inorgchem.4c01009

Empowering Precision C–H Functionalization: Advances in Peroxygenase Engineering

Shuaiqi Meng, Zhongyu Li, Yuncheng Du, Xiao Cheng, Binbin Nian, Siyu Fu, Xiaofei Song and Haiyang Cui

Angewandte Chemie, 2026
DOI: 10.1002/ange.202525086

A Core‐Shell Cascade of Chloroperoxidase and Gold Nanoclusters for Asymmetric Hydroxylation of Ethylbenzene

Meng Xia, Zheyu Wang, Linhan Du, Zhongwang Fu, Guoqiang Jiang, Diannan Lu, Jianzhong Wu and Zheng Liu

ChemCatChem, 2022, 14
DOI: 10.1002/cctc.202101732

Enabling Broader Adoption of Biocatalysis in Organic Chemistry

Evan O. Romero, Anthony T. Saucedo, José R. Hernández-Meléndez, Di Yang, Suman Chakrabarty and Alison R. H. Narayan

JACS Au, 2023, 3, 2073
DOI: 10.1021/jacsau.3c00263

Enantioselective High‐Throughput Assay Showcased for the Identification of (R)‐ as well as (S)‐Selective Unspecific Peroxygenases for C−H Oxidation

Alexander Swoboda, Lukas Johannes Pfeifenberger, Zerina Duhović, Moritz Bürgler, Isabel Oroz‐Guinea, Klara Bangert, Florian Weißensteiner, Lena Parigger, Katharina Ebner, Anton Glieder and Wolfgang Kroutil

Angewandte Chemie, 2023, 135
DOI: 10.1002/ange.202312721

Triaryl Carbenium Ion Pair Mediated Electrocatalytic Benzylic C−H Oxygenation in Air

Zhenguo Zhang, Yongheng Lv, Liang Ji, Peng Chen, Shuyan Han, Yufei Zhu, Lanyang Li, Zhenhua Jia and Teck‐Peng Loh

Angew Chem Int Ed, 2024, 63
DOI: 10.1002/anie.202406588

Catalytic enantioselective C(sp3)–H functionalization involving radical intermediates

Chi Zhang, Zhong-Liang Li, Qiang-Shuai Gu and Xin-Yuan Liu

Nat Commun, 2021, 12
DOI: 10.1038/s41467-020-20770-4