Emerging biosensing platforms based on metal–organic frameworks (MOFs) for detection of exosomes as diagnostic cancer biomarkers: case study for the role of the MOFs

Sofiene Mansouri

J. Mater. Chem. B, 2025, 13, 1586
DOI: 10.1039/D4TB02465F

Extracellular vesicles: from intracellular trafficking molecules to fully fortified delivery vehicles for cancer therapeutics

Adham H. Mohamed, Tasneem Abaza, Yomna A. Youssef, Mona Rady, Sherif Ashraf Fahmy, Rabab Kamel, Nabila Hamdi, Eleni Efthimiado, Maria Braoudaki and Rana A. Youness

Nanoscale Adv., 2025, 7, 934
DOI: 10.1039/D4NA00393D

Fluorescence labeling of extracellular vesicles for diverse bio-applicationsin vitroandin vivo

Yun He, Yanlong Xing, Tongmeng Jiang, Juan Wang, Shenggang Sang, Hong Rong and Fabiao Yu

Chem. Commun., 2023, 59, 6609
DOI: 10.1039/D3CC00998J

Isolation and characterization of extracellular vesicles for clinical applications in cancer – time for standardization?

Nikki Salmond and Karla C. Williams

Nanoscale Adv., 2021, 3, 1830
DOI: 10.1039/D0NA00676A

Calibration and standardization of extracellular vesicle measurements by flow cytometry for translational prostate cancer research

Yohan Kim, Edwin van der Pol, Ali Arafa, Ishwor Thapa, Cameron J. Britton, Jorgena Kosti, Siyang Song, Vidhu B. Joshi, Ree M. Erickson, Hesham Ali and Fabrice Lucien

Nanoscale, 2022, 14, 9781
DOI: 10.1039/D2NR01160C

Separation and isolation of CD9-positive extracellular vesicles from plasma using flow cytometry

Karan Khanna, Nikki Salmond, Sina Halvaei, Andrew Johnson and Karla C. Williams

Nanoscale Adv., 2023, 5, 4435
DOI: 10.1039/D3NA00081H

Identification of DYRK1b as a novel regulator of small extracellular vesicle release using a high throughput nanoscale flow cytometry screening platform

Sina Halvaei, Nikki Salmond and Karla C. Williams

Nanoscale, 2025, 17, 8206
DOI: 10.1039/D4NR02510E

Simultaneous detection of membrane protein and mRNA at single extracellular vesicle level by droplet microfluidics for cancer diagnosis

Huixian Lin, Bo Li, Jingyun Guo, Xueying Mai, Haiyang Yu, Weilun Pan, Bodeng Wu, Wei Liu, Mingzhen Zhong, Tong Liao, Ye Zhang, Bo Situ, Xiaohui Yan, Yifan Liu, Chunchen Liu and Lei Zheng

Journal of Advanced Research, 2025, 73, 645
DOI: 10.1016/j.jare.2024.08.026

Harnessing extracellular vesicle heterogeneity for diagnostic and therapeutic applications

Randy P. Carney, Rachel R. Mizenko, Batuhan T. Bozkurt, Neona Lowe, Tanner Henson, Alessandra Arizzi, Aijun Wang, Cheemeng Tan and Steven C. George

Nat. Nanotechnol., 2025, 20, 14
DOI: 10.1038/s41565-024-01774-3

Sending the Signal to Bone: How Tumor-Derived EVs Orchestrate Pre-Metastatic Niche Formation and Skeletal Colonization

Alhomam Dabaliz, Hagar Mahmoud, Raffi AlMutawa and Khalid S. Mohammad

Biomedicines, 2025, 13, 1640
DOI: 10.3390/biomedicines13071640

Nanomechanical Signatures of Extracellular Vesicles from Hematologic Cancer Patients Unraveled by Atomic Force Microscopy for Liquid Biopsy

Yaqi Feng, Meichen Liu, Xinxin Li, Mi Li, Xiaojing Xing and Lianqing Liu

Nano Lett., 2023, 23, 1591
DOI: 10.1021/acs.nanolett.3c00093

Classification of single extracellular vesicles in a double nanohole optical tweezer for cancer detection

Matthew Peters, Sina Halvaei, Tianyu Zhao, Annie Yang-Schulz, Karla C Williams and Reuven Gordon

J. Phys. Photonics, 2024, 6, 035017
DOI: 10.1088/2515-7647/ad5776

Flow virometry: recent advancements, best practices, and future frontiers

Claire Fernandes, Arvin T. Persaud, Deepa Chaphekhar, Jonathan Burnie, Carolyn Belanger, Vera A. Tang, Christina Guzzo and Herman W. Favoreel

J Virol, 2025, 99
DOI: 10.1128/jvi.01717-24

Extracellular Vesicles as New Players in Drug Delivery: A Focus on Red Blood Cells-Derived EVs

Sara Biagiotti, Faiza Abbas, Mariele Montanari, Chiara Barattini, Luigia Rossi, Mauro Magnani, Stefano Papa and Barbara Canonico

Pharmaceutics, 2023, 15, 365
DOI: 10.3390/pharmaceutics15020365

Clinical significance of STEAP1 extracellular vesicles in prostate cancer

Karan Khanna, Nikki Salmond, Kalan S. Lynn, Hon S. Leong and Karla C. Williams

Prostate Cancer Prostatic Dis, 2021, 24, 802
DOI: 10.1038/s41391-021-00319-2

Extracellular vesicle-based liquid biopsy biomarkers and their application in precision immuno-oncology

Karama Asleh, Valerie Dery, Catherine Taylor, Michelle Davey, Marie-Ange Djeungoue-Petga and Rodney J. Ouellette

Biomark Res, 2023, 11
DOI: 10.1186/s40364-023-00540-2

An aptamer‐guided fluorescence polarisation platform for extracellular vesicle liquid biopsy

Cuong Viet Pham, Rocky Chowdhury, Shweta Patel, Satendra Kumar Jaysawal, Yingchu Hou, Huo Xu, Lee Jia, Yu‐mei Zhang, Xiaowei Wang, Wei Duan and Dongxi Xiang

J of Extracellular Vesicle, 2024, 13
DOI: 10.1002/jev2.12502

Circulating CD81-expressing extracellular vesicles as biomarkers of response for immune-checkpoint inhibitors in advanced NSCLC

Diego Signorelli, Patrizia Ghidotti, Claudia Proto, Marta Brambilla, Alessandro De Toma, Roberto Ferrara, Giulia Galli, Monica Ganzinelli, Giuseppe Lo Russo, Arsela Prelaj, Mario Occhipinti, Giuseppe Viscardi, Valentina Capizzuto, Francesca Pontis, Ilaria Petraroia, Anna Maria Ferretti, Mario Paolo Colombo, Valter Torri, Gabriella Sozzi, Marina Chiara Garassino, Elena Jachetti and Orazio Fortunato

Front. Immunol., 2022, 13
DOI: 10.3389/fimmu.2022.987639

Real‐time monitoring of small extracellular vesicles (sEVs) by in vivo flow cytometry

Fuli Zhang, Xin Lu, Xi Zhu, Ziwen Yu, Weiliang Xia and Xunbin Wei

J of Extracellular Vesicle, 2024, 13
DOI: 10.1002/jev2.70003

Engineering a tunable micropattern‐array assay to sort single extracellular vesicles and particles to detect RNA and protein in situ

Jingjing Zhang, Xilal Y. Rima, Xinyu Wang, Luong T. H. Nguyen, Kristin Huntoon, Yifan Ma, Paola Loreto Palacio, Kim Truc Nguyen, Karunya Albert, Minh‐Dao Duong‐Thi, Nicole Walters, Kwang Joo Kwak, Min Jin Yoon, Hong Li, Jacob Doon‐Ralls, Colin L. Hisey, Daeyong Lee, Yifan Wang, Jonghoon Ha, Kelsey Scherler, Shannon Fallen, Inyoul Lee, Andre F. Palmer, Wen Jiang, Setty M. Magaña, Kai Wang, Betty Y. S. Kim, L. James Lee and Eduardo Reátegui

J of Extracellular Vesicle, 2023, 12
DOI: 10.1002/jev2.12369

Positive and negative selection of extracellular vesicle-based biomarkers: insights from L1CAM immunocapture and emerging immunodepletion strategies

Adam P. S. Bennett

Open Res Europe, 2026, 6, 55
DOI: 10.12688/openreseurope.22603.1

Biophotonics for diagnostic detection of extracellular vesicles

Alice Gualerzi, Silvia Picciolini, Cristiano Carlomagno, Francesca Rodà and Marzia Bedoni

Advanced Drug Delivery Reviews, 2021, 174, 229
DOI: 10.1016/j.addr.2021.04.014

Immunophenotype profile by flow cytometry reveals different subtypes of extracellular vesicles in porcine seminal plasma

Isabel Barranco, Alberto Alvarez-Barrientos, Ana Parra, Pablo Martínez-Díaz, Xiomara Lucas and Jordi Roca

Cell Commun Signal, 2024, 22
DOI: 10.1186/s12964-024-01485-1

Dual-potential electrochemiluminescence cytosensor based on a metal-organic framework and ABEI-PEI-Au@AgNPs for the simultaneous determination of phosphatidylserine and epidermal growth factor receptors on an apoptotic cell surface

Guichun Mo, Dongmiao Qin, Yusheng Wu, Zhi Luo, Keting Mo and Biyang Deng

Microchim Acta, 2023, 190
DOI: 10.1007/s00604-023-05934-5

Monitoring of single extracellular vesicle heterogeneity in cancer progression and therapy

Yoon-Jin Lee, Shinwon Chae and Dongsic Choi

Front. Oncol., 2023, 13
DOI: 10.3389/fonc.2023.1256585

Circulating enolase 1 as a diagnostic biomarker for early-stage breast cancer

Nikki Salmond, Renata Moravcova, Wing Sum Tam, Karan Khanna, Jason C. Rogalski, Kalan Lynn, Muriel Brackstone, Peter H. Watson and Karla C. Williams

npj Precis. Onc., 2025, 9
DOI: 10.1038/s41698-025-01109-y

Microfluidics for separation, detection, and engineering of extracellular vesicles

Lamia A. Heikal, Sherif I. Hamdallah, Salma E. El-Habashy, Asmaa A. Ashour, Riham M. El-Moslemany and Amal H. El-Kamel

Advanced Drug Delivery Reviews, 2026, 231, 115810
DOI: 10.1016/j.addr.2026.115810

Detection of plasma EV-associated TRAIL by nanoscale flow cytometry for liver metastasis prediction in PDAC

Chun-Xiang Huang, Jia-Hong Jian, Jun-Sheng Hao, Zi-Wen Zhou, Zhuo-Qun Li, Dong-Ming Kuang and Cai-Yuan Wu

Adv. Biotechnol., 2026, 4
DOI: 10.1007/s44307-026-00102-1

Nanoprojectile Secondary Ion Mass Spectrometry Enables Multiplexed Analysis of Individual Hepatic Extracellular Vesicles

Seonhwa Lee, Dmitriy S. Verkhoturov, Michael J. Eller, Stanislav V. Verkhoturov, Michael A. Shaw, Kihak Gwon, Yohan Kim, Fabrice Lucien, Harmeet Malhi, Alexander Revzin and Emile A. Schweikert

ACS Nano, 2023, 17, 23584
DOI: 10.1021/acsnano.3c06604

See the Unseen: Rapid, Multiparametric Single‐Particle Analysis of Extracellular Vesicles With Nano‐Flow Cytometry

Yunyun Hu, Qiujin Wu, Junyan Chen, Xin Chen, Haonan Di and Xiaomei Yan

Proteomics, 2025
DOI: 10.1002/pmic.70078

Engineered Extracellular Vesicle-Encapsuled Nanoreactors for Effective Targeting and Cascade Killing of Cancer Cells

Man-Sha Wu, Ze-Rui Zhou, Xiao-Yuan Wang, Jian Lv, Da-Wei Li and Ruo-Can Qian

ACS Appl. Bio Mater., 2023, 6, 1479
DOI: 10.1021/acsabm.2c01019

Shape dynamics of nearly spherical, multicomponent vesicles under shear flow

Anirudh Venkatesh and Vivek Narsimhan

J. Fluid Mech., 2025, 1020
DOI: 10.1017/jfm.2025.10632

Factors to consider before choosing EV labeling method for fluorescence-based techniques

Magdalena Dlugolecka and Malgorzata Czystowska-Kuzmicz

Front. Bioeng. Biotechnol., 2024, 12
DOI: 10.3389/fbioe.2024.1479516

Recent advances of small extracellular vesicle biomarkers in breast cancer diagnosis and prognosis

Yujin Lee, Jie Ni, Julia Beretov, Valerie C. Wasinger, Peter Graham and Yong Li

Mol Cancer, 2023, 22
DOI: 10.1186/s12943-023-01741-x

A magneto-activated nanoscale cytometry platform for molecular profiling of small extracellular vesicles

Kangfu Chen, Bill T. V. Duong, Sharif U. Ahmed, Piriththiv Dhavarasa, Zongjie Wang, Mahmoud Labib, Connor Flynn, Jingya Xu, Yi Y. Zhang, Hansen Wang, Xiaolong Yang, Jagotamoy Das, Hossein Zargartalebi, Yuan Ma and Shana O. Kelley

Nat Commun, 2023, 14
DOI: 10.1038/s41467-023-41285-8

Nanotechnology-driven platforms for extracellular vesicle analysis in tumor immunotherapy

Rui Chen, Qin Kang and Yudong Ning

Front. Immunol., 2025, 16
DOI: 10.3389/fimmu.2025.1632378

Machine learning for extracellular vesicles enables diagnostic and therapeutic nanobiotechnology

Ashutosh Tiwari, Widodo, Dyah Ika Krisnawati, Kai-Yi Tzou and Tsung-Rong Kuo

J Nanobiotechnol, 2026, 24
DOI: 10.1186/s12951-025-03952-4

Preeclampsia Diagnostics and Therapeutics: Advances, Challenges, and Prospects in Nanoscale Exosome-Based Clinical Translation

Md. Akeruzzaman Shaon, Fatema Zerin Farhana, Rushafi Sikder, Kiran Shrestha, Farjana Haque, Milkiyas Toru Tantu, Omar Hamza Bin Manjur, Sharmin Aktar, Kevin M. Koo, Allen G. Ross and Muhammad J. A. Shiddiky

ACS Nano Med., 2026, 1, 342
DOI: 10.1021/acsnanomed.5c00056

Isolation of CD63‐positive epididymosomes from human semen and its application in improving sperm function

Jingwen Luo, Shiqing Zhu, Yafei Kang, Xinyu Liu, Xia Tan, Jieyi Zhao, Xiaofang Ding and Honggang Li

J of Extracellular Vesicle, 2024, 13
DOI: 10.1002/jev2.70006