Issue 32, 2024

Probing action potentials of single beating cardiomyocytes using atomic force microscopy

Abstract

This paper presents a method for using atomic force microscopy to probe action potentials of single beating cardiomyocytes at the nanoscale. In this work, the conductive tip of an atomic force microscope (AFM) was used as a nanoelectrode to record the action potentials of self-beating cardiomyocytes in both the non-constant force contact mode and the constant force contact mode. An electrical model of a tip–cell interface was developed and the indentation force effect on the seal of an AFM conductive tip–cell membrane was theoretically analyzed. The force feedback of AFM allowed for the precise control of tip–cell contact, and enabled reliable measurements. The feasibility of simultaneously recording the action potentials and force information during the contraction of the same beating cardiomyocyte was studied. Furthermore, the AFM tip electrode was used to probe the differences of action potentials using different drugs. This method provides a way at the nanoscale for electrophysiological studies on single beating cardiomyocytes, neurons, and ion channels embedded within the cell membrane in relation to disease states, pharmaceutical drug testing and screening.

Graphical abstract: Probing action potentials of single beating cardiomyocytes using atomic force microscopy

Supplementary files

Article information

Article type
Paper
Submitted
18 Maijs 2024
Accepted
08 Jūl. 2024
First published
09 Jūl. 2024

Anal. Methods, 2024,16, 5527-5535

Probing action potentials of single beating cardiomyocytes using atomic force microscopy

J. Dong, B. Wang, G. Wang, S. Zhang, X. Wang, R. Wang, M. J. C. Crabbe and Z. Wang, Anal. Methods, 2024, 16, 5527 DOI: 10.1039/D4AY00929K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements