Issue 10, 2024

Recent advances in nanopore-based analysis for carbohydrates and glycoconjugates

Abstract

Saccharides are not only the basic constituents and nutrients of living organisms, but also participate in various life activities, and play important roles in cell recognition, immune regulation, development, cancer, etc. The analysis of carbohydrates and glycoconjugates is a necessary means to study their transformations and physiological roles in living organisms. Existing detection techniques can hardly meet the requirements for the analysis of carbohydrates and glycoconjugates in complex matrices as they are expensive, involve complex derivatization, and are time-consuming. Nanopore sensing technology, which is amplification-free and label-free, and is a high-throughput process, provides a new solution for the identification and sequencing of carbohydrates and glycoconjugates. This review highlights recent advances in novel nanopore-based single-molecule sensing technologies for the detection of carbohydrates and glycoconjugates and discusses the advantages and challenges of nanopore sensing technologies. Finally, current issues and future perspectives are discussed with the aim of improving the performance of nanopores in complex media diagnostic applications, as well as providing a new direction for the quantification of glycan chains and the study of glycan chain properties and functions.

Graphical abstract: Recent advances in nanopore-based analysis for carbohydrates and glycoconjugates

Article information

Article type
Critical Review
Submitted
16 Nov. 2023
Accepted
22 Janv. 2024
First published
06 Febr. 2024

Anal. Methods, 2024,16, 1454-1467

Recent advances in nanopore-based analysis for carbohydrates and glycoconjugates

Y. Zhao, Z. Su, X. Zhang, D. Wu, Y. Wu and G. Li, Anal. Methods, 2024, 16, 1454 DOI: 10.1039/D3AY02040A

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