Issue 2, 2024

Recent advances in optical biosensing and imaging of telomerase activity and relevant signal amplification strategies

Abstract

Telomerase as a new valuable biomarker for early diagnosis and prognosis evaluation of cancer has attracted much interest in the field of biosensors, cell imaging, and drug screening. In this review, we mainly focus on different optical techniques and various signal amplification strategies for telomerase activity determination. Fluorometric, colorimetry, chemiluminescence, surface-enhanced Raman scattering (SERS), and dual-mode techniques for telomerase sensing and imaging are summarized. Signal amplification strategies include two categories: one is nucleic acid-based amplification, such as rolling circle amplification (RCA), the hybridization chain reaction (HCR), and catalytic hairpin assembly (CHA); the other is nanomaterial-assisted amplification, including metal nanoclusters, quantum dots, transition metal compounds, graphene oxide, and DNA nanomaterials. Challenges and prospects are also discussed to provide new insights for future development of multifunctional strategies and techniques for in situ and in vivo analysis of biomarkers for accurate cancer diagnosis.

Graphical abstract: Recent advances in optical biosensing and imaging of telomerase activity and relevant signal amplification strategies

Article information

Article type
Critical Review
Submitted
02 Nov. 2023
Accepted
02 Dec. 2023
First published
04 Dec. 2023

Analyst, 2024,149, 290-303

Recent advances in optical biosensing and imaging of telomerase activity and relevant signal amplification strategies

R. Yang, J. Hu, L. Zhang, X. Liu, Y. Huang, L. Zhang and Q. Fan, Analyst, 2024, 149, 290 DOI: 10.1039/D3AN01900D

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