Issue 16, 2022

Highly accurate multiprotein detection on a digital ELISA platform

Abstract

The emerging single-molecule detection platform digital enzyme-linked immunosorbent assay (ELISA) can detect numerous proteins simultaneously at serum concentrations as low as picograms per milliliter. We sought to improve cytokine detection with this platform to aid diagnosis of conditions such as allergy and asthma. We developed a multiple single-molecule detection digital ELISA approach, through the application of encoded magnetic microbeads to simultaneously detect three cytokines in one serum sample. We tested the approach's utility to distinguish asthma-related cytokines in children. Concentrations of interleukin-4 (IL-4) and IL-6 were significantly higher in children with asthma than in healthy controls, while the concentration of interferon-γ (IFN-γ) was significantly lower. Our method has higher accuracy than conventional methods, and our results indicate that the proposed improved high-sensitivity digital ELISA-based diagnosis approach can facilitate early detection and treatment of childhood asthma or related diseases.

Graphical abstract: Highly accurate multiprotein detection on a digital ELISA platform

Article information

Article type
Paper
Submitted
27 Apr. 2022
Accepted
26 Jūn. 2022
First published
27 Jūn. 2022

Lab Chip, 2022,22, 3015-3024

Highly accurate multiprotein detection on a digital ELISA platform

S. Yang, Q. Bi, W. J. Zhang, X. Cui, Y. Zhou, C. Yuan and Y. Cui, Lab Chip, 2022, 22, 3015 DOI: 10.1039/D2LC00388K

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