Issue 11, 2022

Cellular point-of-care diagnostics using an inexpensive layer-stack microfluidic device

Abstract

Cellular analyses are increasingly used to diagnose diseases at point-of-care and global healthcare settings. Some analyses are simple as they rely on chromogenic stains (blood counts, malaria) but others often require higher multiplexing to define and quantitate cell populations (cancer diagnosis, immunoprofiling). Simplifying the latter with inexpensive solutions represents a current bottleneck in designing start-end pipelines. Based on the hypothesis that novel film adhesives could be used to create inexpensive disposable devices, we tested a number of different designs and materials, to rapidly perform 12–15 channel single-cell imaging. Using an optimized passive pumping layer-stack microfluidic (PLASMIC) device (<1 $ in supplies) we show that rapid, inexpensive cellular analysis is feasible.

Graphical abstract: Cellular point-of-care diagnostics using an inexpensive layer-stack microfluidic device

Supplementary files

Article information

Article type
Paper
Submitted
18 Febr. 2022
Accepted
12 Apr. 2022
First published
06 Maijs 2022

Lab Chip, 2022,22, 2145-2154

Cellular point-of-care diagnostics using an inexpensive layer-stack microfluidic device

K. Lucas, J. Oh, J. Hoelzl and R. Weissleder, Lab Chip, 2022, 22, 2145 DOI: 10.1039/D2LC00162D

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