Issue 7, 2022

Development and translation of a paper-based top readout vertical flow assay for SARS-CoV-2 surveillance

Abstract

Surveillance of SARS-CoV-2 infection is critical for controlling the current pandemic. Antigen rapid tests (ARTs) provide a means for surveillance. Available lateral flow assay format ARTs rely heavily on nitrocellulose paper, raising challenges in supply shortage. Vertical flow assay (VFA) with cellulose paper as test material attracts much attention as a complementary test approach. However, current reported VFAs are facing challenges in reading the test signal from the bottom face of the test cassette, complicating the test workflow and hindering translation into rapid test application. Here, we address this gap with an enhanced VFA against SARS-CoV-2 N protein that adapts a cellulose pull-down test format allowing (1) one-step sample application at the top of the test cassette and (2) readout of the test signal from the top. We also demonstrate the feasibility of translating the enhanced VFA into a point-of-care application that can help in SARS-CoV-2 surveillance.

Graphical abstract: Development and translation of a paper-based top readout vertical flow assay for SARS-CoV-2 surveillance

Supplementary files

Article information

Article type
Paper
Submitted
21 Janv. 2022
Accepted
08 Febr. 2022
First published
15 Febr. 2022

Lab Chip, 2022,22, 1321-1332

Development and translation of a paper-based top readout vertical flow assay for SARS-CoV-2 surveillance

H. Jia, E. A. Miller, C. C. Chan, S. Y. Ng, M. Prabakaran, M. Tao, I. S. Cheong, S. M. Lim, M. W. Chen, X. Gao, A. R., M. E. McBee, P. R. Preiser, H. D. Sikes and P. Kongsuphol, Lab Chip, 2022, 22, 1321 DOI: 10.1039/D2LC00073C

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