Issue 48, 2022

Fluorescent probes based on the core–shell structure of molecular imprinted materials and gold nanoparticles for highly selective glutathione detection

Abstract

Glutathione (GSH) is a polypeptide with important physiological functions. Real-time and accurate detection of GSH is of great significance for clinical diagnosis, disease treatment and pathogen detection. A fluorescent nanosensor based on composite core–shell nanoparticles for the highly selective detection of GSH is reported. In the cores, the fluorescence of rhodamine b was quenched by using gold nanoparticles (AuNPs), and GSH could competitively combine with AuNPs to cause rhodamine b to fall off, thereby recovering the fluorescence. In the shell part, molecularly imprinted materials using oxidized glutathione (GSSG) as a pseudotemplate provide GSH/GSSG specific pores and improve the specificity and anti-interference ability of the sensor. The GSH sensor has a detection range of 0–100 μM and limit of detection (LOD) of 0.18 μM, and robust sensing performance in fetal bovine serum, indicating its great potential for clinical diagnosis.

Graphical abstract: Fluorescent probes based on the core–shell structure of molecular imprinted materials and gold nanoparticles for highly selective glutathione detection

Supplementary files

Article information

Article type
Paper
Submitted
23 Aug. 2022
Accepted
14 Nov. 2022
First published
15 Nov. 2022

Anal. Methods, 2022,14, 5034-5040

Fluorescent probes based on the core–shell structure of molecular imprinted materials and gold nanoparticles for highly selective glutathione detection

G. Wu, Y. Zhao, X. Li, X. Lu and T. Qu, Anal. Methods, 2022, 14, 5034 DOI: 10.1039/D2AY01363K

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