Issue 5, 2021

Thin-film-transistor digital microfluidics for high value in vitro diagnostics at the point of need

Abstract

The latest developments in thin-film-transistor digital-microfluidics (TFT-DMF, also known by the commercial name aQdrop™) are reported, and proof of concept application to molecular diagnostics (e.g. for coronavirus disease, COVID-19) at the point-of-need demonstrated. The TFT-DMF array has 41 thousand independently addressable electrodes that are capable of manipulating large numbers of droplets of any size and shape, along any pathway to perform multiple parallel reactions. Droplets are continually tracked and adjusted through closed-loop feedback enabled by TFT based sensors at each array element. The sample-to-answer molecular in vitro diagnostic (IVD) test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) includes nucleic acid extractions from saliva, removal of dsDNA and quantitative reverse transcription polymerase chain reaction (RT-PCR). This proof of concept illustrates how the highly configurable TFT-DMF technology can perform many reactions in parallel and thus support the processing of a range of sample types followed by multiple complex multi-step assays.

Graphical abstract: Thin-film-transistor digital microfluidics for high value in vitro diagnostics at the point of need

Supplementary files

Article information

Article type
Paper
Submitted
12 Nov. 2020
Accepted
29 Dec. 2020
First published
30 Dec. 2020

Lab Chip, 2021,21, 962-975

Thin-film-transistor digital microfluidics for high value in vitro diagnostics at the point of need

S. Anderson, B. Hadwen and C. Brown, Lab Chip, 2021, 21, 962 DOI: 10.1039/D0LC01143F

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