Issue 9, 2021

Direct laser writing for cardiac tissue engineering: a microfluidic heart on a chip with integrated transducers

Abstract

We have developed a microfluidic platform for engineering cardiac microtissues in highly-controlled microenvironments. The platform is fabricated using direct laser writing (DLW) lithography and soft lithography, and contains four separate devices. Each individual device houses a cardiac microtissue and is equipped with an integrated strain actuator and a force sensor. Application of external pressure waves to the platform results in controllable time-dependent forces on the microtissues. Conversely, oscillatory forces generated by the microtissues are transduced into measurable electrical outputs. We demonstrate the capabilities of this platform by studying the response of cardiac microtissues derived from human induced pluripotent stem cells (hiPSC) under prescribed mechanical loading and pacing. This platform will be used for fundamental studies and drug screening on cardiac microtissues.

Graphical abstract: Direct laser writing for cardiac tissue engineering: a microfluidic heart on a chip with integrated transducers

Supplementary files

Article information

Article type
Paper
Submitted
27 Okt. 2020
Accepted
11 Marts 2021
First published
18 Marts 2021

Lab Chip, 2021,21, 1724-1737

Author version available

Direct laser writing for cardiac tissue engineering: a microfluidic heart on a chip with integrated transducers

R. K. Jayne, M. Ç. Karakan, K. Zhang, N. Pierce, C. Michas, D. J. Bishop, C. S. Chen, K. L. Ekinci and A. E. White, Lab Chip, 2021, 21, 1724 DOI: 10.1039/D0LC01078B

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