Issue 3, 2021

ZnO/SiO2 core/shell nanowires for capturing CpG rich single-stranded DNAs

Abstract

Atomic layer deposition (ALD) is capable of providing an ultrathin layer on high-aspect ratio structures with good conformality and tunable film properties. In this research, we modified the surface of ZnO nanowires through ALD for the fabrication of a ZnO/SiO2 (core/shell) nanowire microfluidic device which we utilized for the capture of CpG-rich single-stranded DNAs (ssDNA). Structural changes of the nanowires while varying the number of ALD cycles were evaluated by statistical analysis and their relationship with the capture efficiency was investigated. We hypothesized that finding the optimum number of ALD cycles would be crucial to ensure adequate coating for successful tuning to the desired surface properties, besides promoting a sufficient trapping region with optimal spacing size for capturing the ssDNAs as the biomolecules traverse through the dispersed nanowires. Using the optimal condition, we achieved high capture efficiency of ssDNAs (86.7%) which showed good potential to be further extended for the analysis of CpG sites in cancer-related genes. This finding is beneficial to the future design of core/shell nanowires for capturing ssDNAs in biomedical applications.

Graphical abstract: ZnO/SiO2 core/shell nanowires for capturing CpG rich single-stranded DNAs

Supplementary files

Article information

Article type
Paper
Submitted
19 Nov. 2020
Accepted
18 Dec. 2020
First published
21 Dec. 2020

Anal. Methods, 2021,13, 337-344

Author version available

ZnO/SiO2 core/shell nanowires for capturing CpG rich single-stranded DNAs

M. Musa, T. Yasui, K. Nagashima, M. Horiuchi, Z. Zhu, Q. Liu, T. Shimada, A. Arima, T. Yanagida and Y. Baba, Anal. Methods, 2021, 13, 337 DOI: 10.1039/D0AY02138E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements