Issue 11, 2020

A lysosome-targeted near-infrared fluorescent probe for imaging endogenous cysteine (Cys) in living cells

Abstract

Cysteine (Cys) is one of the most important essential biothiols in lysosomes. Highly selective probes for specific detection and imaging of lysosomal Cys over other biological thiols are rare. Herein, we developed a lysosome-targeted near-infrared fluorescent probe SHCy-C based on a novel NIR-emitting thioxanthene–indolium dye. Due to the turn-on fluorescence response elicited by the intramolecular charge transfer (ICT) processes before and after the reaction with Cys, probe SHCy-C exhibits high selectivity and sensitivity (16 nM) for the detection of Cys. More importantly, probe SHCy-C is found to precisely target lysosomes and achieves the “turn-on” detection and imaging of endogenous Cys in lysosomes.

Graphical abstract: A lysosome-targeted near-infrared fluorescent probe for imaging endogenous cysteine (Cys) in living cells

Supplementary files

Article information

Article type
Paper
Submitted
19 Nov. 2019
Accepted
03 Febr. 2020
First published
04 Febr. 2020

J. Mater. Chem. B, 2020,8, 2269-2274

A lysosome-targeted near-infrared fluorescent probe for imaging endogenous cysteine (Cys) in living cells

S. Cai, C. Liu, X. Jiao, L. Zhao and X. Zeng, J. Mater. Chem. B, 2020, 8, 2269 DOI: 10.1039/C9TB02609F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements