Issue 3, 2020

Engineered assembly of a protein–cucurbituril biohybrid

Abstract

A crystalline biohybrid with a 4 : 1 protein : cucurbituril mass ratio is presented. This result was achieved by engineering additional cucurbit[7]uril (Q7) binding sites into a β-propeller protein. In contrast to the parent protein, Q7-controlled assembly of the engineered variant occurred in solution, as evidenced by NMR and SAXS measurements.

Graphical abstract: Engineered assembly of a protein–cucurbituril biohybrid

Supplementary files

Article information

Article type
Communication
Submitted
13 Sept. 2019
Accepted
04 Dec. 2019
First published
04 Dec. 2019

Chem. Commun., 2020,56, 360-363

Engineered assembly of a protein–cucurbituril biohybrid

F. Guagnini, S. Engilberge, K. O. Ramberg, J. Pérez and P. B. Crowley, Chem. Commun., 2020, 56, 360 DOI: 10.1039/C9CC07198A

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