Issue 21, 2020

Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules

Abstract

Sensors based on fluorogenic RNA aptamers have emerged in recent years. These sensors have been used for in vitro and intracellular detection of a broad range of biological and medical targets. However, the potential application of fluorogenic RNA-based sensors for point-of-care testing is still little studied. Here, we report a paper substrate-based portable fluorogenic RNA sensor system. Target detection can be simply performed by rehydration of RNA sensor-embedded filter papers. This affordable sensor system can be used for the selective, sensitive, and rapid detection of different target analytes, such as antibiotics and cellular signaling molecules. We believe that these paper-based fluorogenic RNA sensors show great potential for point-of-care testing of a wide range of targets from small molecules, nucleic acids, proteins, to various pathogens.

Graphical abstract: Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules

Supplementary files

Article information

Article type
Paper
Submitted
22 Marts 2020
Accepted
06 Maijs 2020
First published
20 Maijs 2020

Anal. Methods, 2020,12, 2674-2681

Author version available

Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules

F. Shafiei, K. McAuliffe, Y. Bagheri, Z. Sun, Q. Yu, R. Wu and M. You, Anal. Methods, 2020, 12, 2674 DOI: 10.1039/D0AY00588F

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