Issue 3, 2019

Advancing single-cell proteomics and metabolomics with microfluidic technologies

Abstract

Recent advances in single-cell analysis have unraveled substantial heterogeneity among seemingly identical cells at genomic and transcriptomic levels. These discoveries have urged scientists to develop new tools that are capable of investigating single cells from a broader set of “omics”. Proteomics and metabolomics, for instance, are of particular interest as they are closely correlated with a dynamic picture of cellular behaviors and phenotypic identities. The development of such tools requires highly efficient isolation and processing of a large number of individual cells, where techniques such as microfluidics are extremely useful. Here, we review the recent advances in single-cell proteomics and metabolomics, with a focus on microfluidics-based platforms. We highlight a vast array of emerging microfluidic formats for single-cell isolation and manipulation, and how the state-of-the-art analytical tools are coupled with such platforms for proteomic and metabolomic profiling.

Graphical abstract: Advancing single-cell proteomics and metabolomics with microfluidic technologies

Article information

Article type
Critical Review
Submitted
05 Aug. 2018
Accepted
29 Sept. 2018
First published
01 Okt. 2018

Analyst, 2019,144, 846-858

Advancing single-cell proteomics and metabolomics with microfluidic technologies

Y. Liu, X. Chen, Y. Zhang and J. Liu, Analyst, 2019, 144, 846 DOI: 10.1039/C8AN01503A

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