Issue 29, 2018

Single-stranded templates as railroad tracks for hierarchical assembly of DNA origami

Abstract

DNA origami is one of the most effective tools for bottom-up construction of novel objects and devices at the nanometer-scale. However, many applications require larger structures than can be obtained with the conventional single-stranded scaffold, typically 7249 nucleotides. Here, we address this limitation by developing custom-made single-stranded scaffolds that bind pre-assembled origami tiles and induce their one-dimensional organization in high yields. Our synthetic method allows the conversion of multiple repetitive and unique sequences into correctly assembled, large backbones, and to finely tune the position and frequency of each building block. Granted with these regions, three and five origami tiles were successfully arranged in 1-D with the aid of one or two scaffolds, forming a nano-“railroad track”. This new method increases length scale in DNA origami without increasing cost and complexity, and is anticipated to increase the yield of other approaches aiming to assemble large origami structures.

Graphical abstract: Single-stranded templates as railroad tracks for hierarchical assembly of DNA origami

Supplementary files

Article information

Article type
Paper
Submitted
18 Apr. 2018
Accepted
24 Jūn. 2018
First published
29 Jūn. 2018

Nanoscale, 2018,10, 13994-13999

Single-stranded templates as railroad tracks for hierarchical assembly of DNA origami

J. F. Rahbani, J. C. C. Hsu, P. Chidchob and H. F. Sleiman, Nanoscale, 2018, 10, 13994 DOI: 10.1039/C8NR03185A

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