Issue 1, 2018

Janus DNA orthogonal adsorption of graphene oxide and metal oxide nanoparticles enabling stable sensing in serum

Abstract

While DNA/graphene oxide (GO) conjugates have been widely used for DNA detection, they suffer from non-specific DNA displacement by proteins, making their application in biological samples difficult. To find new materials tightly adsorbing DNA but not proteins, we screened seven metal oxide nanoparticles, all interacting with the phosphate backbone of DNA, while DNA uses its nucleobases to interact with GO. In this regard, DNA is a Janus polymer orthogonally adsorbing GO and metal oxides. The DNA adsorption affinity ranks CoO > NiO > Cr2O3 > Fe2O3 > Fe3O4 > TiO2 > CeO2 based on a phosphate displacement assay. Among them, CoO is nearly fully resistant to protein displacement, while NiO has the best limit of detection of 0.24 nM DNA. This study provides fundamental insights into the biointerface chemistry of DNA, and reveals new materials useful for bioanalytical chemistry, DNA separation, and DNA-directed assembly.

Graphical abstract: Janus DNA orthogonal adsorption of graphene oxide and metal oxide nanoparticles enabling stable sensing in serum

Supplementary files

Article information

Article type
Communication
Submitted
27 Sept. 2017
Accepted
27 Okt. 2017
First published
27 Okt. 2017

Mater. Horiz., 2018,5, 65-69

Janus DNA orthogonal adsorption of graphene oxide and metal oxide nanoparticles enabling stable sensing in serum

B. Liu, L. Ma, Z. Huang, H. Hu, P. Wu and J. Liu, Mater. Horiz., 2018, 5, 65 DOI: 10.1039/C7MH00804J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements