Issue 5, 2017

Intrinsic blinking of red fluorescent proteins for super-resolution microscopy

Abstract

Single-molecule localization microscopy relies on either controllable photoswitching of fluorescent probes or their robust blinking. We have found that blinking of monomeric red fluorescent proteins TagRFP, TagRFP-T, and FusionRed occurs at moderate illumination power and matches well with camera acquisition speed. It allows for super-resolution image reconstruction of densely labelled structures in live cells using various algorithms.

Graphical abstract: Intrinsic blinking of red fluorescent proteins for super-resolution microscopy

Supplementary files

Article information

Article type
Communication
Submitted
17 Nov. 2016
Accepted
19 Dec. 2016
First published
19 Dec. 2016

Chem. Commun., 2017,53, 949-951

Intrinsic blinking of red fluorescent proteins for super-resolution microscopy

N. V. Klementieva, A. I. Pavlikov, A. A. Moiseev, N. G. Bozhanova, N. M. Mishina, S. A. Lukyanov, E. V. Zagaynova, K. A. Lukyanov and A. S. Mishin, Chem. Commun., 2017, 53, 949 DOI: 10.1039/C6CC09200D

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