Issue 12, 2016

The effects of photodynamic treatment with new methylene blue N on the Candida albicans proteome

Abstract

Candida albicans is a human pathogenic fungus mainly affecting immunocompromised patients. Resistance to the commonly used fungicides can lead to poor treatment of mucosal infections which, in turn, can result in life-threatening systemic candidiasis. In this scenario, antimicrobial photodynamic treatment (PDT) has emerged as an effective alternative to treat superficial and localized fungal infections. Microbial death in PDT is a consequence of the oxidation of many cellular biomolecules, including proteins. Here, we report a combination of two-dimensional electrophoresis and tandem mass spectrometry to study the protein damage resulting from treating C. albicans with PDT with new methylene blue N and red light. Two-dimensional gels of treated cells showed an increase in acidic spots in a fluence-dependent manner. Amino acid analysis revealed a decrease in the histidine content after PDT, which is one plausible explanation for the observed acidic shift. However, some protein spots remained unchanged. Protein identification by mass spectrometry revealed that both modified and unmodified proteins could be localized to the cytoplasm, ruling out subcellular location as the only explanation for damage selectivity. Therefore, we hypothesize that protein modification by PDT is a consequence of both photosensitizer binding affinity and the degree of exposure of the photooxidizable residues on the protein surface.

Graphical abstract: The effects of photodynamic treatment with new methylene blue N on the Candida albicans proteome

Article information

Article type
Paper
Submitted
14 Jūl. 2016
Accepted
19 Okt. 2016
First published
20 Okt. 2016

Photochem. Photobiol. Sci., 2016,15, 1503-1513

The effects of photodynamic treatment with new methylene blue N on the Candida albicans proteome

G. T. P. Brancini, G. B. Rodrigues, M. D. S. L. Rambaldi, C. Izumi, A. P. Yatsuda, M. Wainwright, J. C. Rosa and G. Ú. L. Braga, Photochem. Photobiol. Sci., 2016, 15, 1503 DOI: 10.1039/C6PP00257A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements