Issue 4, 2016

Emerging investigators series: untangling the microbial ecosystem and kinetics in a nitrogen removing photosynthetic high density bioreactor

Abstract

An increasing number of water resource recovery facilities are implementing biological processes for nutrient removal and recovery. One challenge with engineering these processes is the kinetic characterization of nutrient dynamics within microbial communities, where metabolite sharing and varying ecological niches and strategies can lead to complex interactions among organisms. We have applied a 3-dimensional (3-D) visualization method to reveal the effects of varying proportions and total loading of inorganic N species (NH4+ and NO3) on assimilatory and dissimilatory processes by a mixed photosynthetic community within a continuous high density bioreactor (HDBR). This 3-D method enabled the identification of loading conditions that result in maximum specific total N removal rates, which were not easily apparent with 1-dimensional linear regression. Furthermore, microscopic and metagenomic analyses enabled the identification of Chlamydomonas reinhardtii and Parachlorella kessleri as the two dominant algal strains and a member of the Leptolyngbya genus as the dominant cyanobacteria present within the community. Ammonia- and nitrite-oxidizing bacteria (AOB and NOB respectively) were found to comprise a small but significant portion of the bacterial community. Relative and absolute abundance of total bacteria, AOB, NOB, denitrifying bacteria, C. reinhardtii and P. kessleri were obtained from metagenomic and real-time PCR (qPCR) analyses. Within this work, we present evidence that the operational conditions and parameters of a reactor has an effect on each of the investigated components of the microbial community and that those effects ultimately impact the resultant reactor kinetics.

Graphical abstract: Emerging investigators series: untangling the microbial ecosystem and kinetics in a nitrogen removing photosynthetic high density bioreactor

Supplementary files

Article information

Article type
Paper
Submitted
23 Marts 2016
Accepted
19 Apr. 2016
First published
20 Apr. 2016

Environ. Sci.: Water Res. Technol., 2016,2, 705-716

Emerging investigators series: untangling the microbial ecosystem and kinetics in a nitrogen removing photosynthetic high density bioreactor

J. R. Price, S. Keshani Langroodi, Y. Lan, J. M. Becker, W. K. Shieh, G. L. Rosen and C. M. Sales, Environ. Sci.: Water Res. Technol., 2016, 2, 705 DOI: 10.1039/C6EW00078A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements