Issue 47, 2016

Mapping minimum reflection distribution of surface plasmon resonance with a complex refractive index

Abstract

One of the main challenges in making any further advances in the field of SPR biosensing is in the detection of small molecules, low analyte concentrations, and single particle interactions. To this end, labels have been used to amplify the signal of this traditional label-free technique. The physical properties associated with these labels can enhance the SPR signal by affecting the dielectric constant both in the real and imaginary parts. In this paper, we have taken a holistic approach to considering the dielectric properties of these labels and their effect on the minimum resonance intensity (MI) is mapped for the Kretschmann SPR configuration. These maps provide a way for matching label properties for particular SPR conditions and reveal that thicker Au films (∼65 nm) make the MI method more suitable for the detection of absorbing materials. Whereas the wavelength SPR at 50 nm Au film thickness generates a shift of similar or lower magnitude for adsorption of the absorbing label, compared with BSA adsorption, in the ‘thick’-film SPR using the MI format, the signal due to the non-absorbing BSA adsorption tends towards zero (background), whereas the absorbing label produces a large well resolved signal.

Graphical abstract: Mapping minimum reflection distribution of surface plasmon resonance with a complex refractive index

Supplementary files

Article information

Article type
Paper
Submitted
04 Jūl. 2016
Accepted
03 Nov. 2016
First published
04 Nov. 2016

Anal. Methods, 2016,8, 8299-8305

Mapping minimum reflection distribution of surface plasmon resonance with a complex refractive index

X. Hong, M. C. Lapsley, Y. Shang and E. A. H. Hall, Anal. Methods, 2016, 8, 8299 DOI: 10.1039/C6AY01890D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements