Issue 11, 2015

Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents

Abstract

Direct visualization of bioorthogonal alkyne or azide handles using fluorogenic azide–alkyne cycloaddition conducted on the surface of a blot membrane. The method eliminates the need for separation steps to remove excess small molecule reagents before attachment of antigen molecules or other visualization handles, and is especially useful for the analysis of peptides and small proteins. A variety of potential fluorogenic reagents are assessed, and sensitivity (<0.1 picomole) similar to current commercially available fluorescence imaging methods is possible.

Graphical abstract: Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents

Supplementary files

Article information

Article type
Communication
Submitted
29 Jūl. 2015
Accepted
20 Aug. 2015
First published
24 Aug. 2015

Mol. BioSyst., 2015,11, 2846-2849

Author version available

Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents

J. Ohata, F. Vohidov and Z. T. Ball, Mol. BioSyst., 2015, 11, 2846 DOI: 10.1039/C5MB00510H

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