Issue 3, 2010

Quantitative imaging of element spatial distribution in the brain section of a mouse model of Alzheimer's disease using synchrotron radiation X-ray fluorescence analysis

Abstract

A method for quantitative imaging of trace elements in sections of bio-tissues using synchrotron radiation microbeam X-ray fluorescence (SR-μXRF) analysis was developed. The Compton scattering in the SR-μXRF spectrum was utilized as an internal standard to compensate the differences in thickness and density of thin bio-tissue sections. The ratios of element sensitivities to Compton scattering peak obtained from two matrix-matched standard reference materials were used for the calculation of the concentrations of metals in a brain section. The concentrations of Ca, Fe, Cu and Zn in the standard reference material (GBW 08551, pig liver) determined by this method were in good agreement with the certified values. The detection limits of Ca, Fe, Cu and Zn at 2 s detection were 1.15, 0.53, 0.21, and 0.20 μg g−1, respectively. The method has been successfully applied in accurate and precise imaging of the element variations in the brain section of a transgenic mouse model of Alzheimer's disease.

Graphical abstract: Quantitative imaging of element spatial distribution in the brain section of a mouse model of Alzheimer's disease using synchrotron radiation X-ray fluorescence analysis

Article information

Article type
Paper
Submitted
14 Okt. 2009
Accepted
08 Dec. 2009
First published
21 Dec. 2009

J. Anal. At. Spectrom., 2010,25, 328-333

Quantitative imaging of element spatial distribution in the brain section of a mouse model of Alzheimer's disease using synchrotron radiation X-ray fluorescence analysis

H. Wang, M. Wang, B. Wang, X. Meng, Y. Wang, M. Li, W. Feng, Y. Zhao and Z. Chai, J. Anal. At. Spectrom., 2010, 25, 328 DOI: 10.1039/B921201A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements