Issue 19, 2003

Rapid screening by MALDI-TOF mass spectrometry to probe binding specificity at enzyme active sites

Abstract

The binding affinity of aspartate decarboxylase has been probed using MALDI-TOF spectrometry; adducts formed covalently in the active site were detected by MALDI-TOF mass spectrometry after incubation of the enzyme with a range of potential ligands in the presence of NaCNBH3; this has highighted key structural features which will aid design of potential inhibitors.

Graphical abstract: Rapid screening by MALDI-TOF mass spectrometry to probe binding specificity at enzyme active sites

Supplementary files

Article information

Article type
Communication
Submitted
16 Jūl. 2003
Accepted
18 Aug. 2003
First published
01 Sept. 2003

Chem. Commun., 2003, 2416-2417

Rapid screening by MALDI-TOF mass spectrometry to probe binding specificity at enzyme active sites

M. E. Webb, E. Stephens, A. G. Smith and C. Abell, Chem. Commun., 2003, 2416 DOI: 10.1039/B308182F

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