Issue 31, 2024

A multifunctional protein pre-coated metal–organic framework for targeted delivery with deep tissue penetration

Abstract

Targeted drug delivery using metal–organic frameworks (MOFs) has shown significant progress. However, the tumor microenvironment (TME) impedes efficient MOF particle transfer into tumor cells. To tackle this issue, we pre-coated nano-sized MOF-808 particles with multifunctional proteins: glutathione S-transferase (GST)-affibody (Afb) and collagenase, aiming to navigate the TME more effectively. The surface of MOF-808 particles is coated with GST-Afb—a fusion protein of GST and human epidermal growth factor receptor 2 (HER2) Afb or epidermal growth factor receptor (EGFR) Afb which has target affinity. We also added collagenase enzymes capable of breaking down collagen in the extracellular matrix (ECM) through supramolecular conjugation, all without chemical modification. By stabilizing these proteins on the surface, GST-Afb mitigate biomolecule absorption, facilitating specific tumor cell targeting. Simultaneously, collagenase degrades the ECM in the TME, enabling deep tissue penetration of MOF particles. Our resulting system, termed collagenase-GST-Afb-MOF-808 (Col-Afb-M808), minimizes undesired interactions between MOF particles and external biological proteins. It not only induces cell death through Afb-mediated cell-specific targeting, but also showcases advanced cellular internalization in 3D multicellular spheroid cancer models, with effective deep tissue penetration. The therapeutic efficacy of Col-Afb-M808 was further assessed via in vivo imaging and evaluation of tumor inhibition following injection of IR-780 loaded Col-Afb-M808 in 4T1tumor-bearing nude mice. This study offers key insights into the regulation of the multifunctional protein-adhesive surface of MOF particles, paving the way for the designing even more effective targeted drug delivery systems with nano-sized MOF particles.

Graphical abstract: A multifunctional protein pre-coated metal–organic framework for targeted delivery with deep tissue penetration

Supplementary files

Article information

Article type
Paper
Submitted
05 Jūn. 2024
Accepted
17 Jūn. 2024
First published
21 Jūn. 2024
This article is Open Access
Creative Commons BY-NC license

Nanoscale, 2024,16, 14748-14756

A multifunctional protein pre-coated metal–organic framework for targeted delivery with deep tissue penetration

J. Y. Oh, M. Seu, A. K. Barui, H. W. Ok, D. Kim, E. Choi, J. Seong, M. S. Lah and J. Ryu, Nanoscale, 2024, 16, 14748 DOI: 10.1039/D4NR02345E

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