Issue 3, 2024

Advances in colorimetric biosensors of exosomes: novel approaches based on natural enzymes and nanozymes

Abstract

Exosomes are 30–150 nm vesicles derived from diverse cell types, serving as one of the most important biomarkers for early diagnosis and prognosis of diseases. However, the conventional detection method for exosomes faces significant challenges, such as unsatisfactory sensitivity, complicated operation, and the requirement of complicated devices. In recent years, colorimetric exosome biosensors with a visual readout underwent rapid development due to the advances in natural enzyme-based assays and the integration of various types of nanozymes. These synthetic nanomaterials show unique physiochemical properties and catalytic abilities, enabling the construction of exosome colorimetric biosensors with novel principles. This review will illustrate the reaction mechanisms and properties of natural enzymes and nanozymes, followed by a detailed introduction of the recent advances in both types of enzyme-based colorimetric biosensors. A comparison between natural enzymes and nanozymes is made to provide insights into the research that improves the sensitivity and convenience of assays. Finally, the advantages, challenges, and future directions of enzymes as well as exosome colorimetric biosensors are highlighted, aiming at improving the overall performance from different approaches.

Graphical abstract: Advances in colorimetric biosensors of exosomes: novel approaches based on natural enzymes and nanozymes

Article information

Article type
Review Article
Submitted
29 Okt. 2023
Accepted
05 Dec. 2023
First published
20 Dec. 2023

Nanoscale, 2024,16, 1005-1024

Advances in colorimetric biosensors of exosomes: novel approaches based on natural enzymes and nanozymes

Z. Sun, B. Zhang, H. Tu, C. Pan, Y. Chai and W. Chen, Nanoscale, 2024, 16, 1005 DOI: 10.1039/D3NR05459D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements