Issue 2, 2024

Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect

Abstract

Silica NPs (SiNPs) used as a platform to deliver molecules have huge potential for biomedical applications. In order to generate new immunomodulatory tools, 2 variants of SiNPs were synthesized and 3 proteins were adsorbed over their surface: bovine serum albumin and the cytokines IL-1β and TGF-β. Protein adsorption was analyzed according to Langmuir and Freundlich models. The adsorption isotherm of IL-1β on both SiNP variants had a good fit to the Freundlich model, indicating the formation of a protein multilayer around the NPs. For BSA and TGF-β isotherms, the fit to the Langmuir model was better, evidencing the presence of a protein monolayer on the NPs. SiNPs@TGF-β complexes were tested in THP-1 cells (human monocytes cells). The complexes reduced cellular metabolic activity and did not cause an increase in nitric oxide expression, which is related to the immunosuppressive activity of TGF-β, but was potentiated and prolonged over time compared to the cytokine alone. These nanocomplexes could be important tools for use as nanoinmunomodulators (NIMs) for the therapeutic treatment of inflammatory diseases.

Graphical abstract: Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect

Article information

Article type
Paper
Submitted
28 Sept. 2023
Accepted
05 Dec. 2023
First published
05 Dec. 2023
This article is Open Access
Creative Commons BY license

Mater. Adv., 2024,5, 777-787

Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect

E. D. Giorgi, S. Genovés, M. E. Díaz, S. Municoy, M. F. Desimone and M. C. De Marzi, Mater. Adv., 2024, 5, 777 DOI: 10.1039/D3MA00776F

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