Issue 2, 2024

An automated and intelligent microfluidic platform for microalgae detection and monitoring

Abstract

Microalgae not only play a vital role in the ecosystem but also hold promising commercial applications. Conventional methods of detecting and monitoring microalgae rely on field sampling followed by transportation to the laboratory for manual analysis, which is both time-consuming and laborious. Although machine learning (ML) algorithms have been introduced for microalgae detection in the laboratory, no integrated platform approach has yet emerged to enable real-time, on-site sampling and analysing. To solve this problem, here, we develop an automated and intelligent microfluidic platform (AIMP) that can offer automated system control, intelligent data analysis, and user interaction, providing an economical and portable solution to alleviate the drawbacks of conventional methods for microalgae detection and monitoring. We demonstrate the feasibility of the AIMP by detecting and classifying four microalgal species (Cosmarium, Closterium, Micrasterias, and Haematococcus Pluvialis) that exhibit varying sizes (from a few to hundreds of microns) and morphologies. The trained microalgae species detection network (MSDN, based on YOLOv5 architecture) achieves a high overall mean average precision at 0.5 intersection-over-union (mAP@0.5) of 92.8%. Furthermore, the versatility of the AIMP is demonstrated by long-term monitoring of astaxanthin production from Haematococcus Pluvialis over a period of 30 days. The AIMP achieved 97.5% accuracy in the detection of Haematococcus Pluvialis and 96.3% in further classification based on astaxanthin accumulation. This study opens up a new path towards microalgae detection and monitoring using portable intelligent devices, providing new ideas to accelerate progress in the ecological studies and commercial exploitation of microalgae.

Graphical abstract: An automated and intelligent microfluidic platform for microalgae detection and monitoring

Supplementary files

Article information

Article type
Paper
Submitted
07 Okt. 2023
Accepted
03 Dec. 2023
First published
04 Dec. 2023
This article is Open Access
Creative Commons BY license

Lab Chip, 2024,24, 244-253

An automated and intelligent microfluidic platform for microalgae detection and monitoring

J. Zheng, T. Cole, Y. Zhang, Bayinqiaoge, D. Yuan and S. Tang, Lab Chip, 2024, 24, 244 DOI: 10.1039/D3LC00851G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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