Issue 6, 2024

Injectable, shear-thinning, photocrosslinkable, and tissue-adhesive hydrogels composed of diazirine-modified hyaluronan and dendritic polyethyleneimine

Abstract

In the present study, we report the first synthesis of diazirine-modified hyaluronic acid (HA–DAZ). In addition, we also produced a precursor polymer solution composed of HA–DAZ and dendritic polyethyleneimine (DPI) that showed strong shear-thinning properties. Furthermore, its viscosity was strongly reduced (i.e., from 5 × 105 mPa s at 10−3 s−1 to 6 × 101 mPa s at 103 s−1), substantially, which enhanced solution injectability using a 21 G needle. After ultraviolet irradiation at 365 nm and 6 mW cm−2, the HA–DAZ/DPI solution achieved rapid gelation, as measured using the stirring method, and its gelation time decreased from 200 s to 9 s as the total concentrations of HA–DAZ and DPI increased. Following UV irradiation, the storage modulus increased from 40 to 200 Pa. In addition, reversible sol–gel transition and self-healing properties were observed even after UV irradiation. This suggests that the HA–DAZ/DPI hydrogel was crosslinked in multiple ways, i.e., via covalent bonding between the diazirine and amine groups and via intermolecular interactions, including hydrogen bonding, electrostatic interactions, and hydrophobic interactions. A lap shear test showed that the HA–DAZ/DPI hydrogel exhibited strong adhesiveness as a fibrin glue following UV irradiation. Finally, the HA–DAZ/DPI hydrogel showed higher tissue reinforcement than fibrin glue in an ex vivo burst pressure test of the porcine esophageal mucosa.

Graphical abstract: Injectable, shear-thinning, photocrosslinkable, and tissue-adhesive hydrogels composed of diazirine-modified hyaluronan and dendritic polyethyleneimine

Supplementary files

Article information

Article type
Paper
Submitted
03 Aug. 2023
Accepted
21 Dec. 2023
First published
08 Janv. 2024
This article is Open Access
Creative Commons BY-NC license

Biomater. Sci., 2024,12, 1454-1464

Injectable, shear-thinning, photocrosslinkable, and tissue-adhesive hydrogels composed of diazirine-modified hyaluronan and dendritic polyethyleneimine

A. K. S. Chandel, A. Sreedevi Madhavikutty, S. Okada, Z. Qiming, N. F. Inagaki, S. Ohta and T. Ito, Biomater. Sci., 2024, 12, 1454 DOI: 10.1039/D3BM01279D

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