Issue 24, 2024

A fluorescence probe with targeted mitochondria for detecting hydrogen peroxide in vitro and in diabetic mice

Abstract

We designed and prepared probe W-1 for the detection of H2O2. W-1 showed excellent selectivity for H2O2 and was accompanied by colorimetric signal changes. The excellent linear relationship between fluorescence intensity and H2O2 concentration (0–100 μM) provided favorable conditions for its quantitative detection. In addition, the combination of portable test strips with a smartphone platform provided great convenience for on-site visual detection of H2O2. Moreover, W-1 possessed targeting mitochondria property and could be applied to image the exogenous and endogenous H2O2 in cells to distinguish normal cells and cancer cells. Lastly, W-1 was used for monitoring the H2O2 fluctuation of the diabetic process in mice, and the results showed an increase in H2O2 levels in diabetes. Therefore, the probe provided a tool for understanding the pathological and physiological mechanisms of diabetes by imaging H2O2.

Graphical abstract: A fluorescence probe with targeted mitochondria for detecting hydrogen peroxide in vitro and in diabetic mice

Supplementary files

Article information

Article type
Paper
Submitted
10 Apr. 2024
Accepted
20 Maijs 2024
First published
03 Jūn. 2024

Anal. Methods, 2024,16, 3831-3838

A fluorescence probe with targeted mitochondria for detecting hydrogen peroxide in vitro and in diabetic mice

Y. Wang, F. Qiao, Y. Tan, J. Hu, A. Zhang, T. Liang, X. Liu, H. Song and Y. Kang, Anal. Methods, 2024, 16, 3831 DOI: 10.1039/D4AY00653D

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