Issue 28, 2024

Mass spectrometry-intensive top-down proteomics: an update on technology advancements and biomedical applications

Abstract

Proteoforms are all forms of protein molecules from the same gene because of variations at the DNA, RNA, and protein levels, e.g., alternative splicing and post-translational modifications (PTMs). Delineation of proteins in a proteoform-specific manner is crucial for understanding their biological functions. Mass spectrometry (MS)-intensive top-down proteomics (TDP) is promising for comprehensively characterizing intact proteoforms in complex biological systems. It has achieved substantial progress in technological development, including sample preparation, proteoform separations, MS instrumentation, and bioinformatics tools. In a single TDP study, thousands of proteoforms can be identified and quantified from a cell lysate. It has also been applied to various biomedical research to better our understanding of protein function in regulating cellular processes and to discover novel proteoform biomarkers of diseases for early diagnosis and therapeutic development. This review covers the most recent technological development and biomedical applications of MS-intensive TDP.

Graphical abstract: Mass spectrometry-intensive top-down proteomics: an update on technology advancements and biomedical applications

Article information

Article type
Critical Review
Submitted
09 Apr. 2024
Accepted
25 Jūn. 2024
First published
27 Jūn. 2024
This article is Open Access
Creative Commons BY license

Anal. Methods, 2024,16, 4664-4682

Mass spectrometry-intensive top-down proteomics: an update on technology advancements and biomedical applications

T. Xu, Q. Wang, Q. Wang and L. Sun, Anal. Methods, 2024, 16, 4664 DOI: 10.1039/D4AY00651H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements