Issue 14, 2024

Response strategies and biological applications of organic fluorescent thermometry: cell- and mitochondrion-level detection

Abstract

Temperature homeostasis is critical for cells to perform their physiological functions. Among the diverse methods for temperature detection, fluorescent temperature probes stand out as a proven and effective tool, especially for monitoring temperature in cells and suborganelles, with a specific emphasis on mitochondria. The utilization of these probes provides a new opportunity to enhance our understanding of the mechanisms and interconnections underlying various physiological activities related to temperature homeostasis. However, the complexity and variability of cells and suborganelles necessitate fluorescent temperature probes with high resolution and sensitivity. To meet the demanding requirements for intracellular/subcellular temperature detection, several strategies have been developed, offering a range of options to address this challenge. This review examines four fundamental temperature-response strategies employed by small molecule and polymer probes, including intramolecular rotation, polarity sensitivity, Förster resonance energy transfer, and structural changes. The primary emphasis was placed on elucidating molecular design and biological applications specific to each type of probe. Furthermore, this review provides an insightful discussion on factors that may affect fluorescent thermometry, providing valuable perspectives for future development in the field. Finally, the review concludes by presenting cutting-edge response strategies and research insights for mitigating biases in temperature sensing.

Graphical abstract: Response strategies and biological applications of organic fluorescent thermometry: cell- and mitochondrion-level detection

Article information

Article type
Minireview
Submitted
18 Janv. 2024
Accepted
06 Marts 2024
First published
06 Marts 2024
This article is Open Access
Creative Commons BY license

Anal. Methods, 2024,16, 1968-1984

Response strategies and biological applications of organic fluorescent thermometry: cell- and mitochondrion-level detection

S. Li, Y. Li, S. Zhang, H. Fang, Z. Huang, D. Zhang, A. Ding, K. Uvdal, Z. Hu, K. Huang and L. Li, Anal. Methods, 2024, 16, 1968 DOI: 10.1039/D4AY00117F

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