Issue 14, 2024

Sandwich-type aptamer-based biosensors for thrombin detection

Abstract

Thrombin, a proteolytic enzyme, plays an essential role in catalyzing many blood clotting reactions. Thrombin can act as a marker for some blood-related diseases, such as leukemia, thrombosis, Alzheimer's disease and liver disease. Therefore, its diagnosis is of great importance in the fields of biological and medical research. Biosensors containing sandwich-type structures have attracted much consideration owing to their superior features such as reproducible and stable responses with easy improvement in the sensitivity of detection. Sandwich-type platforms can be designed using a pair of receptors that are able to bind to diverse locations of the same target. Herein, we investigate recent advances in the progress and applications of thrombin aptasensors containing a sandwich-type structure, in which two thrombin-binding aptamers (TBAs) identify different parts of the thrombin molecule, leading to the formation of a sandwich structure and ultimately signal detection. We also discuss the pros and cons of these approaches and outline the most logical approach in each section.

Graphical abstract: Sandwich-type aptamer-based biosensors for thrombin detection

Article information

Article type
Critical Review
Submitted
08 Dec. 2023
Accepted
12 Febr. 2024
First published
16 Febr. 2024

Anal. Methods, 2024,16, 1985-2001

Sandwich-type aptamer-based biosensors for thrombin detection

S. Sahraneshin Samani, E. Sameiyan, F. Tabatabaei Yazdi, S. A. Mortazavi, M. Alibolandi, M. Ramezani, S. M. Taghdisi and K. Abnous, Anal. Methods, 2024, 16, 1985 DOI: 10.1039/D3AY02196C

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