Issue 10, 2024

Application of CRISPR/Cas13a-based biosensors in serum marker detection

Abstract

The detection of serum markers is important for the early diagnosis and monitoring of diseases, but conventional detection methods have the problem of low specificity or sensitivity. CRISPR/Cas13a-based biosensors have the characteristics of simple detection methods and high sensitivity, which have a certain potential to solve the problems of conventional detection. This paper focuses on the research progress of CRISPR/Cas13a-based biosensors in serum marker detection, introduces the principles and applications of fluorescence, electrochemistry, colorimetric, and other biosensors based on CRISPR/Cas13a in the detection of serum markers, compares and analyzes the differences between the above CRISPR/Cas13a-based biosensors, and looks forward to the future development direction of CRISPR/Cas13a-based biosensors.

Graphical abstract: Application of CRISPR/Cas13a-based biosensors in serum marker detection

Article information

Article type
Critical Review
Submitted
31 Okt. 2023
Accepted
31 Janv. 2024
First published
22 Febr. 2024

Anal. Methods, 2024,16, 1426-1438

Application of CRISPR/Cas13a-based biosensors in serum marker detection

W. He, X. Liu, J. Na, H. Bian, L. Zhong and G. Li, Anal. Methods, 2024, 16, 1426 DOI: 10.1039/D3AY01927F

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