Issue 47, 2023

Calixarene-based cryoprotectants for ice recrystallization inhibition and cell cryopreservation

Abstract

The development of new cryoprotectants for cryopreservation of cells has attracted considerable interest. Herein, five calixarene-based CPAs (SC4A, S-S-C4A, S-SO2-C4A, SBAC4A, and CAC4A) were developed, and their IRI activity, DIS property and cryoprotective effect were studied. SBAC4A with a sulphobetaine zwitterion and SC4A with sulfo group modification possessed better cryoprotective effects than the other calixarene-based CPAs, especially for SBAC4A with the enhanced cell viabilities of 16.16 ± 1.78%, 12.60 ± 1.15% and 14.90 ± 1.66% against MCF-7, hucMSCs and A549 cells, respectively. This result provides a supramolecular principle for developing novel CPAs with consideration of the factors of hydrogen bonding, the macromolecular crowding principle and the three-dimensional (3D) structure.

Graphical abstract: Calixarene-based cryoprotectants for ice recrystallization inhibition and cell cryopreservation

Supplementary files

Article information

Article type
Communication
Submitted
17 Okt. 2023
Accepted
18 Nov. 2023
First published
20 Nov. 2023

J. Mater. Chem. B, 2023,11, 11222-11227

Calixarene-based cryoprotectants for ice recrystallization inhibition and cell cryopreservation

B. Hu, J. Li, Y. Ren, T. Zhang, L. Chen, X. Li, D. Guo and K. Wang, J. Mater. Chem. B, 2023, 11, 11222 DOI: 10.1039/D3TB02432F

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