Issue 36, 2023

In vitro evaluation of granules obtained from 3D sphene scaffolds and bovine bone grafts: chemical and biological assays

Abstract

Sphene is an innovative bone graft material. The aim of this study was to investigate and compare the physicochemical and biological properties of Bio-Oss® (BO) and in-lab synthesized and processed sphene granules. BO granules of 1000–2000 μm (BO-L), 250–1000 μm (BO-S) and 100–200 μm (BO-p) for derived granules, and corresponding groups of sphene granules obtained from 3D printed blocks (SB-L, SB-S, SB-p) and foams (SF-L, SF-S and SF-p) were investigated. The following analyses were conducted: morphological analysis, specific surface area and porosity, inductively coupled plasma mass spectrometry (ICP-MS), cytotoxicity assay, Alizarin staining, bone-related gene expression, osteoblast migration and proliferation assays. All pulverized granules exhibited a similar morphology and SF-S resembled natural bone. Sphene-derived granules showed absence of micro- and mesopores and a low specific surface area. ICP-MS revealed a tendency for absorption of Ca and P for all BO samples, while sphene granules demonstrated a release of Ca. No cellular cytotoxicity was detected and osteoblastic phenotype in primary cells was observed, with significantly increased values for SF-L, SF-S, BO-L and BO-p. Further investigations are needed before clinical use can be considered.

Graphical abstract: In vitro evaluation of granules obtained from 3D sphene scaffolds and bovine bone grafts: chemical and biological assays

  • This article is part of the themed collection: #MyFirstJMCB

Supplementary files

Article information

Article type
Paper
Submitted
08 Marts 2023
Accepted
27 Aug. 2023
First published
30 Aug. 2023
This article is Open Access
Creative Commons BY license

J. Mater. Chem. B, 2023,11, 8775-8787

In vitro evaluation of granules obtained from 3D sphene scaffolds and bovine bone grafts: chemical and biological assays

S. Sivolella, G. Brunello, E. Nika, D. Badocco, P. Pastore, S. M. Carturan, E. Bernardo, H. Elsayed, L. Biasetto and P. Brun, J. Mater. Chem. B, 2023, 11, 8775 DOI: 10.1039/D3TB00499F

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