Issue 33, 2023

Role of mutations in a chemoenzymatic enantiodivergent C(sp3)–H insertion: exploring the mechanism and origin of stereoselectivity

Abstract

New-to-nature enzymes have emerged as powerful catalysts in recent years for streamlining various stereoselective organic transformations. While synthetic strategies employing engineered enzymes have witnessed proliferating success, there is limited clarity on the mechanistic front and more so when considering molecular-level insights into the role of selected mutations, dramatically escalating catalytic competency and selectivity. We have investigated the mechanism and correlation between mutations and exquisite stereoselectivity of a lactone carbene insertion into the C(sp3)–H bond of substituted aniline, catalyzed by two mutants of a cytochrome P450 variant, “P411” (engineered through directed evolution) in which the axial cysteine has been mutated to serine, utilizing various computational tools. The pivotal role of S264 and L/R328 mutations in the active site has been delineated computationally using two cluster models, thus rationalizing the enantiodivergence. This report provides much-needed insights into the origin of enantiodivergence, furnishing a mechanistic framework for understanding the anchoring effects of H-bond donor residues with the lactone ring. This study is expected to have important implications in the rational design of stereodivergent enzymes and toward successful in silico enzyme designing.

Graphical abstract: Role of mutations in a chemoenzymatic enantiodivergent C(sp3)–H insertion: exploring the mechanism and origin of stereoselectivity

Supplementary files

Article information

Article type
Edge Article
Submitted
01 Jūn. 2023
Accepted
23 Jūl. 2023
First published
25 Jūl. 2023
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2023,14, 8810-8822

Role of mutations in a chemoenzymatic enantiodivergent C(sp3)–H insertion: exploring the mechanism and origin of stereoselectivity

R. Chatterjee and G. Jindal, Chem. Sci., 2023, 14, 8810 DOI: 10.1039/D3SC02788K

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