Issue 41, 2023

Covalent functionalisation controlled by molecular design for the aptameric recognition of serotonin in graphene-based field-effect transistors

Abstract

In the last decade, solution-gated graphene field effect transistors (GFETs) showed their versatility in the development of a miniaturized multiplexed platform for electrophysiological recordings and sensing. Due to their working mechanism, the surface functionalisation and immobilisation of receptors are pivotal to ensure the proper functioning of devices. Herein, we present a controlled covalent functionalisation strategy based on molecular design and electrochemical triggering, which provide a monolayer-like functionalisation of micro-GFET arrays retaining the electronic properties of graphenes. The functionalisation layer as a receptor was then employed as the linker for serotonin aptamer conjugation. The micro-GFET arrays display sensitivity toward the target analyte in the micromolar range in a physiological buffer (PBS 10 mM). The sensor allows the in-flow real-time monitoring of serotonin transient concentrations with fast and reversible responses.

Graphical abstract: Covalent functionalisation controlled by molecular design for the aptameric recognition of serotonin in graphene-based field-effect transistors

Supplementary files

Article information

Article type
Paper
Submitted
18 Aug. 2023
Accepted
19 Sept. 2023
First published
19 Sept. 2023
This article is Open Access
Creative Commons BY license

Nanoscale, 2023,15, 16650-16657

Covalent functionalisation controlled by molecular design for the aptameric recognition of serotonin in graphene-based field-effect transistors

C. Wetzl, S. Brosel-Oliu, M. Carini, D. Di Silvio, X. Illa, R. Villa, A. Guimera, E. Prats-Alfonso, M. Prato and A. Criado, Nanoscale, 2023, 15, 16650 DOI: 10.1039/D3NR04153K

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