Issue 5, 2023

Recent methods of droplet microfluidics and their applications in spheroids and organoids

Abstract

Droplet microfluidic techniques have long been known as a high-throughput approach for cell manipulation. The capacity to compartmentalize cells into picolitre droplets in microfluidic devices has opened up a range of new ways to extract information from cells. Spheroids and organoids are crucial in vitro three-dimensional cell culture models that physiologically mimic natural tissues and organs. With the aid of developments in cell biology and materials science, droplet microfluidics has been applied to construct spheroids and organoids in numerous formats. In this article, we divide droplet microfluidic approaches for managing spheroids and organoids into three categories based on the droplet module format: liquid droplet, microparticle, and microcapsule. We discuss current advances in the use of droplet microfluidics for the generation of tumour spheroids, stem cell spheroids, and organoids, as well as the downstream applications of these methods in high-throughput screening and tissue engineering.

Graphical abstract: Recent methods of droplet microfluidics and their applications in spheroids and organoids

Article information

Article type
Critical Review
Submitted
31 Maijs 2022
Accepted
07 Nov. 2022
First published
11 Janv. 2023

Lab Chip, 2023,23, 1080-1096

Recent methods of droplet microfluidics and their applications in spheroids and organoids

Y. Wang, M. Liu, Y. Zhang, H. Liu and L. Han, Lab Chip, 2023, 23, 1080 DOI: 10.1039/D2LC00493C

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