Issue 14, 2022

One-step electrochemical deposition of antifouling polymers with pyrogallol for biosensing applications

Abstract

Electrochemical techniques are highly sensitive and label-free sensing methods for the detection of various biomarkers, toxins, or pathogens. An ideal sensing element should be electroconductive, nonfouling, and readily available for conjugation of ligands. In this work, we have developed a facile, one-step electrodeposition method based on pyrogallol polymerization for preparation of a nonfouling and biotinylated surface on indium tin oxide (ITO). A copolymer of sulfobetaine methacrylate and aminoethyl methacrylate (pSBAE) was synthesized and deposited on ITO in the presence of pyrogallol via cyclic voltammetry. The deposition took less than 15 minutes to sufficiently inhibit cell adhesion. Using biotinylated pSBAE, the modified surface resisted nonspecific protein adsorption from the fetal bovine serum solution and detected added avidin concentrations. The results show an efficient platform to fabricate an electrochemical biosensor for the detection of biomarkers. We expect that this facile one-step technology could be applied to conjugate various biosensing elements for nonfouling biosensors.

Graphical abstract: One-step electrochemical deposition of antifouling polymers with pyrogallol for biosensing applications

Article information

Article type
Paper
Submitted
18 Nov. 2021
Accepted
27 Dec. 2021
First published
31 Dec. 2021

J. Mater. Chem. B, 2022,10, 2504-2511

One-step electrochemical deposition of antifouling polymers with pyrogallol for biosensing applications

S. Yeh, P. Deval, J. Wu, S. Luo and W. Tsai, J. Mater. Chem. B, 2022, 10, 2504 DOI: 10.1039/D1TB02536H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements