Issue 47, 2022

An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting

Abstract

The ability to regulate, maintain and reproduce fluorogenic properties is a fundamental prerequisite of modern molecular diagnostics, nanotechnology and bioimaging. The sequence-dependence of the fluorescence properties in fluorophores commonly used in nucleic acid labelling is here being exploited to assemble a color scale in 256 shades of green Cy3 fluorescence. Using photolithography, we synthesize microarrays of labeled nucleic acids that can accurately reproduce 8-bit monochrome graphics by mapping color to fluorescence intensity and sequence. This DNA-based painting approach paves the way for a full RGB scale array fabrication process.

Graphical abstract: An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting

Supplementary files

Article information

Article type
Communication
Submitted
24 Sept. 2022
Accepted
17 Nov. 2022
First published
18 Nov. 2022
This article is Open Access
Creative Commons BY license

Nanoscale, 2022,14, 17528-17533

An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting

T. Kekić and J. Lietard, Nanoscale, 2022, 14, 17528 DOI: 10.1039/D2NR05269E

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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