Issue 38, 2022

Visualization of intercellular cargo transfer using upconverting nanoparticles

Abstract

Cell–cell communication is important for cellular differentiation, organ function, and immune responses. In intercellular communication, the extracellular vesicles (EVs) play a significant role in delivering the cargo molecules such as genes, proteins, and enzymes, to regulate and control the ability of the recipient cells. In this study, the observation of intercellular cargo transfer via dual-colour imaging using upconverting nanoparticles (UCNPs) has been demonstrated. Using this technique, the intercellular transport via contact-dependent and contact-independent signaling in live HeLa cells was clearly visualized with real-time, long-term single-vesicle tracking. Furthermore, it was demonstrated that the endocytosed UCNPs can be transmitted with the encapsulation of EVs labelled with fluorescent proteins.

Graphical abstract: Visualization of intercellular cargo transfer using upconverting nanoparticles

Associated articles

Supplementary files

Article information

Article type
Communication
Submitted
12 Apr. 2022
Accepted
11 Aug. 2022
First published
11 Aug. 2022

Nanoscale, 2022,14, 14008-14013

Visualization of intercellular cargo transfer using upconverting nanoparticles

Y. Goh, J. Kim, H. S. Park, T. Jung, K. S. Hong, S. H. Nam, Y. D. Suh and K. T. Lee, Nanoscale, 2022, 14, 14008 DOI: 10.1039/D2NR01999J

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