Issue 7, 2022

Optimization of a CE-ICP-MS/MS method for the investigation of liposome–cisplatin nanosystems and their interactions with transferrin

Abstract

As chemotherapy suffers from the limitation of non-selectivity towards cancer cells, resulting in severe side effects, the targeted delivery of anticancer drugs such as cisplatin using nontoxic nanomaterials has been under extensive examination. In such an approach, qualities such as biocompatibility, biodegradability, ease of synthesis (including the surface modifying possibility with targeting ligands), and the tunable encapsulation of chemotherapeutics make liposomes superior to other nanomaterials as drug nanocarriers. Despite ten liposome-drug formulations being approved for the market, none of them concern platinum-based anticancer drugs. This can be due to the problematic multistep synthesis of such systems and the use of ineffective analytical tools for their characterization. Consequently, the current study aims to propose a straightforward protocol for liposome–cisplatin system synthesis as well as a comprehensive method for their characterization based on the combination of capillary electrophoresis (CE) and inductively coupled plasma-tandem mass spectrometry (ICP-MS/MS). Although the application of CE-ICP-MS has been reported in previous studies on liposome–cisplatin systems, the advantages of the optimized method proposed in our study include not only the possibility of the direct quantitative monitoring of liposome–metallodrug systems (all analytes of interest) but also their interactions with proteins in close-to-physiological conditions of analysis due to the applied tandem mass mode of the spectrometer. The present approach thereby facilitates overcoming the limitations of the strategies previously described in the literature, especially in the case of sulfur monitoring.

Graphical abstract: Optimization of a CE-ICP-MS/MS method for the investigation of liposome–cisplatin nanosystems and their interactions with transferrin

Associated articles

Supplementary files

Article information

Article type
Technical Note
Submitted
23 Dec. 2021
Accepted
01 Jūn. 2022
First published
01 Jūn. 2022
This article is Open Access
Creative Commons BY-NC license

J. Anal. At. Spectrom., 2022,37, 1442-1449

Optimization of a CE-ICP-MS/MS method for the investigation of liposome–cisplatin nanosystems and their interactions with transferrin

A. M. Wróblewska, J. Samsonowicz-Górski, E. Kamińska, M. Drozd and M. Matczuk, J. Anal. At. Spectrom., 2022, 37, 1442 DOI: 10.1039/D1JA00459J

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements