Issue 24, 2022

Specific detection of fusion transcripts based on a duplex-specific nuclease and isothermal exponential amplification reaction

Abstract

Fusion genes are mostly found in tumor tissues, but are in low expression levels in healthy tissues, making them good candidate biomarkers for tumor diagnosis and therapy. Here, we propose a duplex-specific nuclease-isothermal exponential amplification reaction (DSN-IEXPAR) method for the detection of fusion transcripts. A DNA probe is specifically designed for fusion transcript recognition and hybridization, and DSN cleavages the DNA probe in the DNA/RNA duplex. Through controlling the recognition and cleavage temperature, DSN can only cut the DNA probe fully matched with the target fusion transcript rather than other transcripts containing partial the same sequence, endowing the proposed method with high specificity to the fusion transcript in the presence of homologous sequences. The truncated DNA probe after cutting can subsequently trigger IEXPAR as a probe, so as low as 100 fM fusion transcript can be detected with the proposed DSN-IEXPAR. The evaluation of the analytical performance of DSN-IEXPAR demonstrates that it can provide an effective platform for fusion transcript detection in the ordinary laboratory and clinical diagnosis.

Graphical abstract: Specific detection of fusion transcripts based on a duplex-specific nuclease and isothermal exponential amplification reaction

Supplementary files

Article information

Article type
Paper
Submitted
11 Okt. 2022
Accepted
01 Nov. 2022
First published
02 Nov. 2022

Analyst, 2022,147, 5649-5654

Specific detection of fusion transcripts based on a duplex-specific nuclease and isothermal exponential amplification reaction

F. Su, T. Li, X. He and Z. Li, Analyst, 2022, 147, 5649 DOI: 10.1039/D2AN01665F

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