Issue 31, 2021

GNPS-guided discovery of xylacremolide C and D, evaluation of their putative biosynthetic origin and bioactivity studies of xylacremolide A and B

Abstract

Targeted HRMS2-GNPS-based metabolomic analysis of Pseudoxylaria sp. X187, a fungal antagonist of the fungus-growing termite symbiosis, resulted in the identification of two lipopeptidic congeners of xylacremolides, named xylacremolide C and D, which are built from D-phenylalanine, L-proline and an acetyl-CoA starter unit elongated by four malonyl-CoA derived ketide units. The putative xya gene cluster was identified from a draft genome generated by Illumina and PacBio sequencing and RNAseq studies. Biological activities of xylacremolide A and B were evaluated and revealed weak histone deacetylase inhibitory (HDACi) and antifungal activities, as well as moderate protease inhibition activity across a panel of nine human, viral and bacterial proteases.

Graphical abstract: GNPS-guided discovery of xylacremolide C and D, evaluation of their putative biosynthetic origin and bioactivity studies of xylacremolide A and B

Supplementary files

Article information

Article type
Paper
Submitted
05 Febr. 2021
Accepted
10 Maijs 2021
First published
24 Maijs 2021
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2021,11, 18748-18756

GNPS-guided discovery of xylacremolide C and D, evaluation of their putative biosynthetic origin and bioactivity studies of xylacremolide A and B

F. Schalk, J. Fricke, S. Um, B. H. Conlon, H. Maus, N. Jäger, T. Heinzel, T. Schirmeister, M. Poulsen and C. Beemelmanns, RSC Adv., 2021, 11, 18748 DOI: 10.1039/D1RA00997D

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