Issue 23, 2021

Asparaginyl endopeptidases: enzymology, applications and limitations

Abstract

Asparaginyl endopeptidases (AEP) are cysteine proteases found in mammalian and plant cells. Several AEP isoforms from plant species were found to exhibit transpeptidase activity which is integral for the key head-to-tail cyclisation reaction during the biosynthesis of cyclotides. Since many plant AEPs exhibit excellent enzyme kinetics for peptide ligation via a relatively short substrate recognition sequence, they have become appealing tools for peptide and protein modification. In this review, research focused on the enzymology of AEPs and their applications in polypeptide cyclisation and labelling will be presented. Importantly, the limitations of using AEPs and opportunities for future research and innovation will also be discussed.

Graphical abstract: Asparaginyl endopeptidases: enzymology, applications and limitations

Article information

Article type
Review Article
Submitted
30 Marts 2021
Accepted
12 Maijs 2021
First published
12 Maijs 2021
This article is Open Access
Creative Commons BY license

Org. Biomol. Chem., 2021,19, 5048-5062

Asparaginyl endopeptidases: enzymology, applications and limitations

T. M. S. Tang and L. Y. P. Luk, Org. Biomol. Chem., 2021, 19, 5048 DOI: 10.1039/D1OB00608H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements