Issue 4, 2021

One-pot fluorescent assay for sensitive detection of APOBEC3A activity

Abstract

We reported a one-pot fluorescence-based assay to quantitively detect A3A activity combined with cytosine deamination and uracil excision. After deamination by A3A and USER enzyme treatment, the fluorescent turn-on effect at 520 nm was observed, which can be used to evaluate the A3A activity and screen inhibitors.

Graphical abstract: One-pot fluorescent assay for sensitive detection of APOBEC3A activity

Supplementary files

Article information

Article type
Communication
Submitted
05 Apr. 2021
Accepted
30 Apr. 2021
First published
30 Apr. 2021
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2021,2, 1201-1205

One-pot fluorescent assay for sensitive detection of APOBEC3A activity

B. Wang, X. Zhang, Y. Wang, K. Chen, F. Wang, X. Weng and X. Zhou, RSC Chem. Biol., 2021, 2, 1201 DOI: 10.1039/D1CB00076D

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